Prostaglandin modulation of N-formylmethionylleucylphenylalanine-induced transmembrane potential changes in rat neutrophils
dc.contributor.author | Fantone, Joseph C. | en_US |
dc.contributor.author | Duque, Ricardo E. | en_US |
dc.contributor.author | Phan, Sem H. | en_US |
dc.date.accessioned | 2006-04-07T18:25:48Z | |
dc.date.available | 2006-04-07T18:25:48Z | |
dc.date.issued | 1984-07-20 | en_US |
dc.identifier.citation | Fantone, Joseph C., Duque, Ricardo E., Phan, Sem H. (1984/07/20)."Prostaglandin modulation of N-formylmethionylleucylphenylalanine-induced transmembrane potential changes in rat neutrophils." Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 804(3): 265-274. <http://hdl.handle.net/2027.42/24755> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T20-47S651G-KM/2/e5f38ce94d7dd220bee2c06d872e8e06 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/24755 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6331525&dopt=citation | en_US |
dc.description.abstract | Prostaglandins of the E-series (PGEs) and PGI2 will inhibit formylmethionylleucylphenylalanine- (f-Met-Leu-Phe) induced lysosomal enzyme release and superoxide-anion (O-2) production by neutrophils. The inhibitory effects of PGEs and PGI2 on neutrophil functional responses have been correlated with their ability to increase intracellular cAMP. In this study we have examined the effects of PGEs and PGI2 on f-Met-Leu-Phe- and phorbol-myristate-acetate-induced rat neutrophil membrane potential changes using an optical probe of membrane potential 3,3-dipropylthiodicarbocyanine iodide. 15-(S)-15-methyl-PGE1 (15-methyl-PGE1), a stable analogue of PGE1 and PGI2 inhibited f-Met-Leu-Phe-induced transmembrane potential changes in a dose-dependent manner. This inhibition was correlated with the ability of these agents to increase intracellular cAMP levels and inhibit O-2 production and degranulation. In contrast, 15-methyl-PGE1 and PGI2, did not inhibit phorbol-myristate-acetate-induced transmembrane potential changes and O-2 production. These results suggest independent mechanisms of activation of neutrophils by phorbol myristate acetate and f-Met-Leu-Phe, and they also suggest that the inhibitory effects of prostaglandins and cAMP on f-Met-Leu-Phe-stimulated cells is at a step or steps prior to activation of those processes involved in effecting changes in transmembrane potential, which are common to both stimuli. | en_US |
dc.format.extent | 748163 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Prostaglandin modulation of N-formylmethionylleucylphenylalanine-induced transmembrane potential changes in rat neutrophils | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 6331525 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/24755/1/0000177.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0167-4889(84)90129-0 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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