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In vitro colony formation by cryopreserved primary human tumor cells
dc.contributor.authorAllen, Edward D.en_US
dc.contributor.authorGau, T. C.en_US
dc.contributor.authorNatale, Robert B.en_US
dc.date.accessioned2006-04-07T18:27:04Z
dc.date.available2006-04-07T18:27:04Z
dc.date.issued1984-06en_US
dc.identifier.citationAllen, E. D., Gau, T. C., Natale, R. B. (1984/06)."In vitro colony formation by cryopreserved primary human tumor cells." Cryobiology 21(2): 240-245. <http://hdl.handle.net/2027.42/24790>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WD5-4F1J0TV-B4/2/0d948efb61f77ed301f1b8e2f91baf2den_US
dc.identifier.urihttps://hdl.handle.net/2027.42/24790
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6713950&dopt=citationen_US
dc.description.abstractThe effect of cryopreservation on the ability of primary human cancer cells to form colonies in a two-layer agar system was examined. Although considerable variation occurred, concentrations of 5 or 10% dimethylsulfoxide employed with slow freezing rates allowed survival of colonies in the range 20-40% or greater of nonfrozen controls. The methods used in this study do not require elaborate freezing equipment, and can be used for the cryopreservation of a wide variety of types of cancers.en_US
dc.format.extent417827 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleIn vitro colony formation by cryopreserved primary human tumor cellsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelStatistics and Numeric Dataen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMathematicsen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelSocial Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Internal Medicine, Division of Hematology/Oncology, University of Michigan, Ann Arbor, Michigan 48109, U.S.A.en_US
dc.contributor.affiliationumDepartment of Internal Medicine, Division of Hematology/Oncology, University of Michigan, Ann Arbor, Michigan 48109, U.S.A.; Medical Research, Veterans Administration Medical Center, Ann Arbor, U.S.A.en_US
dc.contributor.affiliationotherMedical Research, Veterans Administration Medical Center, Ann Arbor, U.S.A.en_US
dc.identifier.pmid6713950en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/24790/1/0000216.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0011-2240(84)90215-3en_US
dc.identifier.sourceCryobiologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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