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Highly sensitive and rapid gene mapping using miniaturized blot hybridization: application to prenatal diagnosis
dc.contributor.authorLaw, David J.en_US
dc.contributor.authorFrossard, Philippe M.en_US
dc.contributor.authorRucknagel, Donald L.en_US
dc.date.accessioned2006-04-07T18:28:21Z
dc.date.available2006-04-07T18:28:21Z
dc.date.issued1984-05en_US
dc.identifier.citationLaw, David J., Frossard, Philippe M., Rucknagel, Donald L. (1984/05)."Highly sensitive and rapid gene mapping using miniaturized blot hybridization: application to prenatal diagnosis." Gene 28(2): 153-158. <http://hdl.handle.net/2027.42/24827>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T39-47T2H45-16/2/663dbd7df334f101ad040c6d66b6f759en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/24827
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6735178&dopt=citationen_US
dc.description.abstractWe have developed a protocol for the preparation and analysis of amniocyte DNA which permits more sensitive and more rapid antenatal detection of sickle-cell anemia (SCA) than previously has been possible. After rapid extraction of DNA from amniotic cells, only 50 ng of MstII-digested DNA need be analyzed by mini-gel electrophoresis and hybridization detection to determine reliably the fetal genotype. Under these conditions, the entire gene-mapping procedure can be performed within 5 days. When larger amounts of DNA (&gt; 500 ng) are analyzed, the minimal diagnosis time is reduced to 2 days. The resolution of restriction fragments on mini-gels is comparable to that obtained with larger gels. The 1.15-kb [beta]A and 1.35-kb [beta]S MstII fragments are well separated. The technique is useful whenever rapid and sensitive analysis of genomic DNA is desired.en_US
dc.format.extent627102 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleHighly sensitive and rapid gene mapping using miniaturized blot hybridization: application to prenatal diagnosisen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelGeneticsen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Human Genetics, University of Michigan Medical School, Ann Arbor,USAen_US
dc.contributor.affiliationumDepartment of Human Genetics, University of Michigan Medical School, Ann Arbor,USAen_US
dc.contributor.affiliationumDepartment of Human Genetics, University of Michigan Medical School, Ann Arbor,USAen_US
dc.identifier.pmid6735178en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/24827/1/0000253.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0378-1119(84)90252-Xen_US
dc.identifier.sourceGeneen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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