Reexamination of the carbohydrate binding stoichiometry of lima bean lectin
dc.contributor.author | Roberts, David D. | en_US |
dc.contributor.author | Goldstein, Irwin J. | en_US |
dc.date.accessioned | 2006-04-07T18:29:22Z | |
dc.date.available | 2006-04-07T18:29:22Z | |
dc.date.issued | 1984-04 | en_US |
dc.identifier.citation | Roberts, David D., Goldstein, Irwin J. (1984/04)."Reexamination of the carbohydrate binding stoichiometry of lima bean lectin." Archives of Biochemistry and Biophysics 230(1): 316-320. <http://hdl.handle.net/2027.42/24856> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DW3D37-17C/2/c8a0fde6f92ba430ca9d0036f68a28b7 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/24856 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6712240&dopt=citation | en_US |
dc.description.abstract | The carbohydrate binding stoichiometry of lima bean lectin component III was reexamined using equilibrium dialysis and quantitative affinity chromatography following limited chemical modification. Equilibrium dialysis employing methyl[2-14C]benzamido-2-deoxy-[alpha]--galactopyranoside as ligand demonstrated that the lectin tetramer bound 4 mol of sugar with Kassoc = 1.44 +/- 0.13 x 103 m-1 (T = 5 [deg]C, pH 7.0, ionic strength 0.1). The previous report of two sites/tetramer [Bessler, W. and Goldstein, I. J. (1974) Arch. Biochem. Biophys. 165, 444] appears to be the result of partial inactivation of the lectin due to oxidation of essential thiol groups. Following limited chemical modification of the thiol groups by methyl methanethiosulfonate, multiple intermediate forms with reduced affinity for Synsorb A were obtained. The number and hemagglutinating activities of these intermediates provided further support for the presence of four carbohydrate binding sites on lima bean lectin component III. | en_US |
dc.format.extent | 459645 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Reexamination of the carbohydrate binding stoichiometry of lima bean lectin | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 6712240 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/24856/1/0000283.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(84)90113-9 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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