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Enzyme-linked lectin assay (ELLA) : II. Detection of carbohydrate groups on the surface of unfixed cells

dc.contributor.authorMcCoy, J. Philip Jr.en_US
dc.contributor.authorVarani, Jamesen_US
dc.contributor.authorGoldstein, Irwin J.en_US
dc.date.accessioned2006-04-07T18:30:10Z
dc.date.available2006-04-07T18:30:10Z
dc.date.issued1984-03en_US
dc.identifier.citationMcCoy, Jr, J. Philip, Varani, James, Goldstein, Irwin J. (1984/03)."Enzyme-linked lectin assay (ELLA) : II. Detection of carbohydrate groups on the surface of unfixed cells." Experimental Cell Research 151(1): 96-103. <http://hdl.handle.net/2027.42/24879>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WFC-4DM1VW9-7G/2/ad5387bc52c10ad76dc1d2041b34d6been_US
dc.identifier.urihttps://hdl.handle.net/2027.42/24879
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6698125&dopt=citationen_US
dc.description.abstractAn enzyme-linked lectin assay (ELLA) has been developed to detect specific carbohydrate units on the surface of unfixed cells. The assay may be read in standard ELISA plate readers, since the cell-bound enzyme-lectin conjugate is specifically eluted from the cells prior to development of the conjugate. ELLA, when read in an enzyme-linked immunosorbent assay (ELISA) plate reader, allows better detection and relative quantitation of specific surface carbohydrate units than is possible by standard immunofluorescence with fluorescein-conjugated lectins.en_US
dc.format.extent1340319 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleEnzyme-linked lectin assay (ELLA) : II. Detection of carbohydrate groups on the surface of unfixed cellsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartments of Pathology and Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartments of Pathology and Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartments of Pathology and Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USAen_US
dc.identifier.pmid6698125en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/24879/1/0000306.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0014-4827(84)90359-8en_US
dc.identifier.sourceExperimental Cell Researchen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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