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Dissociation constants for carbonic anhydrase-sulfonamide binding by high-performance liquid chromatography

dc.contributor.authorOsborne, William R. A.en_US
dc.contributor.authorTashian, Richard E.en_US
dc.date.accessioned2006-04-07T18:30:33Z
dc.date.available2006-04-07T18:30:33Z
dc.date.issued1984-03en_US
dc.identifier.citationOsborne, William R. A., Tashian, Richard E. (1984/03)."Dissociation constants for carbonic anhydrase-sulfonamide binding by high-performance liquid chromatography." Analytical Biochemistry 137(2): 302-306. <http://hdl.handle.net/2027.42/24890>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DYM9JV-12S/2/84c4a8f371730605a284af5457c43f31en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/24890
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6428265&dopt=citationen_US
dc.description.abstractCarbonic anhydrase-azosulfonamide dissociation constants (Kd) were determined by gel filtration with high-performance liquid chromatography. By measuring the area of the elution peak at two wavelengths. Kd values were derived without having to measure a shallow trough. The procedure proved to be fast and reliable and has a general application. The dissociation constants were measured for 7-acetamido-2-(4'-sulfamylphenylazo)-1-hydroxynaphthalene-3,6-disulfonate (Neoprontosil) complexes with carbonic anhydrase isozymes CA I, CA II, and CA III from bovine and human sources, and chicken CA III from skeletal muscle.en_US
dc.format.extent410915 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleDissociation constants for carbonic anhydrase-sulfonamide binding by high-performance liquid chromatographyen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationotherDepartment of Pediatrics, RD-20, University of Washington, Seattle, Washington 98195, USAen_US
dc.identifier.pmid6428265en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/24890/1/0000317.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(84)90089-7en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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