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The distribution of calmodulin in living mitotic cells
dc.contributor.authorZavortink, Michaelen_US
dc.contributor.authorWelsh, Michael J.en_US
dc.contributor.authorMcIntosh, J. Richarden_US
dc.date.accessioned2006-04-07T18:36:22Z
dc.date.available2006-04-07T18:36:22Z
dc.date.issued1983-12en_US
dc.identifier.citationZavortink, Michael, Welsh, Michael J., McIntosh, J. Richard (1983/12)."The distribution of calmodulin in living mitotic cells." Experimental Cell Research 149(2): 375-385. <http://hdl.handle.net/2027.42/25048>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WFC-4DKKF6D-6/2/4b4bd5ba208ee4b93cf1123c2979f9bden_US
dc.identifier.urihttps://hdl.handle.net/2027.42/25048
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6641808&dopt=citationen_US
dc.description.abstractCalmodulin has been labeled with rhodamine isothiocyanate (CaM-RITC) and used as a probe for the location of calmodulin in vivo. CaM-RITC retains its capacity to regulate the activity of brain phosphodiesterase in a Ca2+-dependent manner in vitro, indicating that the labeled protein is still active. After injection into living mammalian cells CaM-RITC incorporates rapidly into the mitotic spindle; the details of its localization there mimic closely the distribution of Calmodulin seen by immunofluorescence. In interphase cells the CaM-RITC is excluded from the nucleus, but shows no region of specific concentration within the cytoplasm. Neither a 2-fold increase in cellular CaM nor the injection of anti CaM has any observable effect on the progress of mitosis.en_US
dc.format.extent5737218 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleThe distribution of calmodulin in living mitotic cellsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Anatomy and Cell Biology, School of Medicine, University of Michigan, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationotherDepartment of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USAen_US
dc.contributor.affiliationotherDepartment of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USAen_US
dc.identifier.pmid6641808en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/25048/1/0000476.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0014-4827(83)90350-6en_US
dc.identifier.sourceExperimental Cell Researchen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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