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Electron microscopic demonstration of horseradish peroxidase-tetramethylbenzidine reaction product as a method for identifying sensory nerve fibers in the rat tooth pulp

dc.contributor.authorTurner, Dennis F.en_US
dc.contributor.authorMarfurt, Carl F.en_US
dc.date.accessioned2006-04-07T18:36:56Z
dc.date.available2006-04-07T18:36:56Z
dc.date.issued1983-11-11en_US
dc.identifier.citationF. Turner, Dennis, F. Marfurt, Carl (1983/11/11)."Electron microscopic demonstration of horseradish peroxidase-tetramethylbenzidine reaction product as a method for identifying sensory nerve fibers in the rat tooth pulp." Neuroscience Letters 41(3): 213-217. <http://hdl.handle.net/2027.42/25061>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T0G-485GHCM-8C/2/7722d8ad70bcae215f876ea661025adcen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/25061
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6664612&dopt=citationen_US
dc.description.abstractThe purpose of the present investigation was to determine if the horseradish peroxidase could be used as a method for labeling sensory nerve fibers (specifically, tooth pulp afferents) for detailed ultrastructural analyses. HRP injected into the trigeminal ganglion of adult rats was taken up by ganglion cell bodies and transported anterogradely to their peripheral endings in the dental tissues. Following perfusion-fixation, the teeth were decalcified in EDTA, sectioned, reacted for HRP activity according to the tetramethylbenzidine (TMB) technique, and processed for electron microscopy. The HRP-TMB reaction product was clearly visible within most of the axons in the dental pulp, appearing as conspicuous, rectangular shaped aggregates of fine rods or needles.en_US
dc.format.extent962919 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleElectron microscopic demonstration of horseradish peroxidase-tetramethylbenzidine reaction product as a method for identifying sensory nerve fibers in the rat tooth pulpen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelPsychologyen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelSocial Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Oral Biology, University of Michigan Dental School, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationotherDepartment of Anatomy, The Milton S. Hershey Medical Center, The Pennsylvania State University, Hershey, PA 17033, U.S.A.en_US
dc.identifier.pmid6664612en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/25061/1/0000489.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0304-3940(83)90453-6en_US
dc.identifier.sourceNeuroscience Lettersen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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