View Item 

Show simple item record

Transfer of small plasmid DNA fragments from polyacrylamide gels onto nitrocellulose paper
dc.contributor.authorFrossard, Philippe M.en_US
dc.contributor.authorSisco, Kenneth L.en_US
dc.contributor.authorRucknagel, Donald L.en_US
dc.date.accessioned2006-04-07T18:37:52Z
dc.date.available2006-04-07T18:37:52Z
dc.date.issued1983-10-15en_US
dc.identifier.citationFrossard, Philippe, Sisco, Kenneth L., Rucknagel, Donald L. (1983/10/15)."Transfer of small plasmid DNA fragments from polyacrylamide gels onto nitrocellulose paper." Analytical Biochemistry 134(2): 265-268. <http://hdl.handle.net/2027.42/25088>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DW3N23-87/2/ae8c9d6c5f6b7ba44eaa7a412d5944d0en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/25088
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6316804&dopt=citationen_US
dc.description.abstractThe incorporation into a 7% polyacrylamide gel of a nucleic acid-specific photochemical reagent 4,5',8-trimethylpsoralen at a concentration of 1 mg/dl of acrylamide solution improves both the sensitivity and the efficiency of the transfer of plasmid DNA fragments from the gel onto nitrocellulose filters. This improvement allows detection by blot hybridization of DNA fragments as small as 57 bp long.en_US
dc.format.extent1055901 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleTransfer of small plasmid DNA fragments from polyacrylamide gels onto nitrocellulose paperen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Internal Medicine, Simpson Memorial Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Department of Human Genetics, Simpson Memorial Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Internal Medicine, Simpson Memorial Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Department of Human Genetics, Simpson Memorial Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Internal Medicine, Simpson Memorial Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Department of Human Genetics, Simpson Memorial Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid6316804en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/25088/1/0000519.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(83)90295-6en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
0000519.pdf
Size:
1.006MB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.