Separation of hemoglobin types by cation-exchange high-performance liquid chromatography,
dc.contributor.author | Gupta, Shri P. | en_US |
dc.contributor.author | Hanash, Samir M. | en_US |
dc.date.accessioned | 2006-04-07T18:38:08Z | |
dc.date.available | 2006-04-07T18:38:08Z | |
dc.date.issued | 1983-10-01 | en_US |
dc.identifier.citation | Gupta, Shri P., Hanash, Samir M. (1983/10/01)."Separation of hemoglobin types by cation-exchange high-performance liquid chromatography,." Analytical Biochemistry 134(1): 117-121. <http://hdl.handle.net/2027.42/25096> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-4DW3N6C-B6/2/a9b950ae444f947dfc1962336cb91a2c | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25096 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6660481&dopt=citation | en_US |
dc.description.abstract | The use of a recently developed cation-exchange HPLC packing material for the separation of hemoglobin types in human blood has been investigated. Adult and newborn hemolysates from normal individuals and from subjects with hemoglobin disorders were analyzed using a weak cation carboxymethyl-bonded phase on 5-[mu]m-particle-size silica. Elution was accomplished using a Bistris (2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-1,3-propanediol) gradient. Seven well-resolved HbA1 fractions eluted before the major HbA peak. Hbs A1a, A1b, A1c and an HbA1 fraction that increased with aging of the hemolysates were separately eluted. HbF when present or when added to the hemolysates eluted as a distinct peak. HbA was followed by Hbs A2, S, and C when present. An early-eluting peak corresponding to Hb Bart's was identified in newborn hemolysates. It is concluded that cation-exchange HPLC provides a new tool for the reliable separation of minor hemoglobin components. | en_US |
dc.format.extent | 410283 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Separation of hemoglobin types by cation-exchange high-performance liquid chromatography, | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics, University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Pediatrics, University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 6660481 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25096/1/0000528.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2697(83)90271-3 | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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