Insulin resistance in H-35 rat hepatoma cells is mediated by post-receptor mechanisms
dc.contributor.author | Krett, Nancy L. | en_US |
dc.contributor.author | Heaton, Joanne H. | en_US |
dc.contributor.author | Gelehrter, Thomas D. | en_US |
dc.date.accessioned | 2006-04-07T18:38:47Z | |
dc.date.available | 2006-04-07T18:38:47Z | |
dc.date.issued | 1983-09 | en_US |
dc.identifier.citation | Krett, Nancy L., Heaton, Joanne H., Gelehrter, Thomas D. (1983/09)."Insulin resistance in H-35 rat hepatoma cells is mediated by post-receptor mechanisms." Molecular and Cellular Endocrinology 32(1): 91-100. <http://hdl.handle.net/2027.42/25115> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T3G-47RB4MC-N6/2/a8c112c5b7d456981f7b1823d4c2645c | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25115 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6138288&dopt=citation | en_US |
dc.description.abstract | Incubation of H-35 cells with 300 ng/ml (50 nM) of insulin causes a 3-4-fold induction of tyrosine aminotransferase at 4-6 h of incubation. At 24 h the activity of transaminase returns to basal levels despite the presence of sufficient insulin to stimulate a maximal response. Furthermore, addition of 300 ng/ml of fresh insulin fails to stimulate the induction of transaminase. In contrast, the addition of 0.1 [mu]M dexamethasone to insulintreated cells stimulates the induction of tyrosine aminotransferase, indicating that the loss of responsiveness is specific to insulin action. Incubation of H-35 cells with insulin also causes a 25-30% decrease in insulin binding. This modest decrease in receptor binding is not sufficient to explain the virtually complete loss of insulin responsiveness. Hence, in H-35 hepatoma cells insulin-induced desensitization to insulin action is mediated primarily by post-receptor events. | en_US |
dc.format.extent | 657648 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Insulin resistance in H-35 rat hepatoma cells is mediated by post-receptor mechanisms | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Human Genetics and Internal Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Human Genetics and Internal Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Human Genetics and Internal Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 6138288 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25115/1/0000548.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0303-7207(83)90101-6 | en_US |
dc.identifier.source | Molecular and Cellular Endocrinology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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