Evidence that a nucleotide sequence, "boxA," is involved in the action of the NusA protein
dc.contributor.author | Friedman, David I. | en_US |
dc.contributor.author | Olson, Eric R. | en_US |
dc.date.accessioned | 2006-04-07T18:39:46Z | |
dc.date.available | 2006-04-07T18:39:46Z | |
dc.date.issued | 1983-08 | en_US |
dc.identifier.citation | Friedman, David I., Olson, Eric R. (1983/08)."Evidence that a nucleotide sequence, "boxA," is involved in the action of the NusA protein." Cell 34(1): 143-149. <http://hdl.handle.net/2027.42/25144> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WSN-4C8FYN6-1X/2/73bd99e1150537ad83ca3214198bbdf6 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25144 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6309406&dopt=citation | en_US |
dc.description.abstract | We report the isolation of a mutation, boxA 1, in the nutR region of the phage [lambda] genome. The nutR region, located downstream of the pR promoter, includes the site nutR where the [lambda] N protein is thought to act to render subsequent transcription termination-resistant. We have previously suggested that the boxA sequence, 5'CGCTCTTA3' (or its RNA analog), located 8 bp promoter-proximal to nutR, might be the recognition site for the E. coli host factor, NusA, which has been shown to be necessary for N action. The boxA1 mutation, an A:T to T:A transversion, results in a changed boxA sequence upstream of nutR, CGCTCTTT. This change is necessary for [lambda] to effectively use the NusA of Salmonella typhimurium, a NusA function not normally active with the N product of [lambda]. Other lambdoid phages with unique N functions and nut sites that are normally active with the NusA of Salmonella have boxA sequences with the terminal three Ts. Moreover, sequences closely resembling boxA have been found near transcription termination sequences in E. coli operons where NusA has been shown to be involved in termination. These findings identify boxA as an important recognition signal for the NusA protein. | en_US |
dc.format.extent | 1221314 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Evidence that a nucleotide sequence, "boxA," is involved in the action of the NusA protein | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology The University of Michigan School of Medicine, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology The University of Michigan School of Medicine, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 6309406 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25144/1/0000580.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0092-8674(83)90144-7 | en_US |
dc.identifier.source | Cell | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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