Haploid accumulation and translational control of phosphoglycerate kinase-2 messenger RNA during mouse spermatogenesis
dc.contributor.author | Gold, Bert | en_US |
dc.contributor.author | Fujimoto, Hirokazu | en_US |
dc.contributor.author | Kramer, James M. | en_US |
dc.contributor.author | Erickson, Robert P. | en_US |
dc.contributor.author | Hecht, Norman B. | en_US |
dc.date.accessioned | 2006-04-07T18:40:05Z | |
dc.date.available | 2006-04-07T18:40:05Z | |
dc.date.issued | 1983-08 | en_US |
dc.identifier.citation | Gold, Bert, Fujimoto, Hirokazu, Kramer, James M., Erickson, Robert P., Hecht, Norman B. (1983/08)."Haploid accumulation and translational control of phosphoglycerate kinase-2 messenger RNA during mouse spermatogenesis." Developmental Biology 98(2): 392-399. <http://hdl.handle.net/2027.42/25153> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WDG-4DV03XV-G/2/c5023d96a2e7ad2d2485c26844550126 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25153 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6873461&dopt=citation | en_US |
dc.description.abstract | The intracellular location of the mRNA for the testis-specific isozyme of phosphoglycerate kinase-2 (PGK-2) has been determined for two spermatogenic cell types. The mRNA activity for PGK-2 from the polysomal and nonpolysomal fractions of pachytene primary spermatocytes or round spermatids has been assayed by cell-free translation with the polypeptide products monitored by immunoprecipitation, followed by one-dimensional or two-dimensional electrophoresis and fluorography. The results reveal that the majority of PGK-2 mRNA activity of round spermatids was present in the polysomal fraction while the relatively less abundant PGK-2 mRNA of pachytene primary spermatocytes was present in the nonpolysomal fraction. No PGK-2 mRNA activity was observed in the cytoplasmic RNA from primitive type A spermatogonia or prepubertal Sertoli cells. These data indicate that mature PGK-2 mRNA first appears in the cytoplasm of spermatogenic cells during the prophase of meiosis and increases in amount after meiosis. Although mature PGK-2 mRNA is present in meiotic cells it is not actively translated until after meiosis has been completed. Thus, mRNA accumulation and translational mechanisms are involved in the control of phosphoglycerate kinase-2 synthesis during spermatogenesis. | en_US |
dc.format.extent | 3128098 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Haploid accumulation and translational control of phosphoglycerate kinase-2 messenger RNA during mouse spermatogenesis | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, Box 015, University of Michigan School of Medicine, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Human Genetics, Box 015, University of Michigan School of Medicine, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Human Genetics, Box 015, University of Michigan School of Medicine, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Tufts University, Department of Biology, Medford, Massachusetts 02155, USA | en_US |
dc.contributor.affiliationother | Tufts University, Department of Biology, Medford, Massachusetts 02155, USA | en_US |
dc.identifier.pmid | 6873461 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25153/1/0000589.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0012-1606(83)90368-8 | en_US |
dc.identifier.source | Developmental Biology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.