Immunocytochemistry of the C-terminal peptide of propressophysin (CPP): Relationship to vasopressin, oxytocin and neurophysin
dc.contributor.author | Watson, Stanley J. | en_US |
dc.contributor.author | Khachaturian, Henry | en_US |
dc.contributor.author | Seidah, Nabil G. | en_US |
dc.contributor.author | Chretien, Michel | en_US |
dc.contributor.author | Zimmerman, Earl | en_US |
dc.contributor.author | Nilaver, Gajanan | en_US |
dc.contributor.author | van Wimersma Gredianus, T. B. | en_US |
dc.date.accessioned | 2006-04-07T18:40:56Z | |
dc.date.available | 2006-04-07T18:40:56Z | |
dc.date.issued | 1983-07 | en_US |
dc.identifier.citation | Watson, Stanley J., Khachaturian, Henry, Seidah, Nabil G., Chretien, Michel, Zimmerman, Earl, Nilaver, Gajanan, van Wimersma Gredianus, T. B. (1983/07)."Immunocytochemistry of the C-terminal peptide of propressophysin (CPP): Relationship to vasopressin, oxytocin and neurophysin." Neuropeptides 3(5): 321-336. <http://hdl.handle.net/2027.42/25176> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WNR-4C4WD3K-2G/2/ca5e199b5f4743ef094d895804829121 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25176 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6194471&dopt=citation | en_US |
dc.description.abstract | Arginine-vasopressin (AVP) and its associated neurophysin (AVP-NP) are synthesized via a precursor, propressophysin, which also contains a 39 amino acid glycopeptide at its C-terminus (C-terminus of propressophysin, or CPP). In the present study, immunocytochemical techniques were used to determine the cellular co-localization of CPP with AVP, oxytocin (OXY), AVP-NP and OXY-NP in the rat hypothalamus using colchicine pre-treatment and serial 5 [mu]m section analysis. Extensive cross-competition studies of antisera raised against each peptide with the various antigens yielded no significant crossreactivity of the CPP, AVP, OXY and NP antisera. The NP antiserum, although directed against both AVP-NP and OXY-NP, demonstrated a preference for OXY-NP at a dilution of 1:20,000. CPP and AVP were always co-localized within the same magnocellular neurons of the supraoptic, paraventricular and circularis nuclei, and further showed very similar patterning in the suprachiasmatic nucleus as well. In conrast, no cellular overlap could be detected between CPP and OXY, in any of the above nuclei (the suprachiasmatic nucleus is devoid of OXY). Likewise, no examples of co-localization of CPP and OXY-NP were found in the magnocellular nuclei. These results are in strong agreement with a biosynthetic relationship between CPP, AVP and AVP-NP, and their separateness from the OXY and OXY-NP precursor. | en_US |
dc.format.extent | 1608053 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Immunocytochemistry of the C-terminal peptide of propressophysin (CPP): Relationship to vasopressin, oxytocin and neurophysin | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationother | Protein and Pituitary Hormone Laboratory, Clinical Research Institute of Montreal, Montreal, Canada | en_US |
dc.contributor.affiliationother | Protein and Pituitary Hormone Laboratory, Clinical Research Institute of Montreal, Montreal, Canada | en_US |
dc.contributor.affiliationother | College of Physicians and Surgeons of Columbia University, Dept. of Neurology, New York, New York, USA | en_US |
dc.contributor.affiliationother | College of Physicians and Surgeons of Columbia University, Dept. of Neurology, New York, New York, USA | en_US |
dc.contributor.affiliationother | Rudolf Magnus Institute for Pharmacology, State University of Utrecht, Utrecht, The Netherlands | en_US |
dc.identifier.pmid | 6194471 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25176/1/0000615.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0143-4179(83)90021-5 | en_US |
dc.identifier.source | Neuropeptides | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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