Comparison of six rabbit liver cytochrome P-450 isozymes in formation of a reactive metabolite of acetaminophen
dc.contributor.author | Morgan, Edward T. | en_US |
dc.contributor.author | Koop, Dennis R. | en_US |
dc.contributor.author | Coon, Minor J. | en_US |
dc.date.accessioned | 2006-04-07T18:43:23Z | |
dc.date.available | 2006-04-07T18:43:23Z | |
dc.date.issued | 1983-04-15 | en_US |
dc.identifier.citation | Morgan, Edward T., Koop, Dennis R., Coon, Minor J. (1983/04/15)."Comparison of six rabbit liver cytochrome P-450 isozymes in formation of a reactive metabolite of acetaminophen." Biochemical and Biophysical Research Communications 112(1): 8-13. <http://hdl.handle.net/2027.42/25240> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4DYVHVW-M4/2/692e718166819d39f6366c5fc27dc5e4 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25240 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6838622&dopt=citation | en_US |
dc.description.abstract | This laboratory has recently reported the isolation of an ethanol-inducible form of rabbit liver microsomal cytochrome P-450, designated isozyme 3a. In view of the reports of others that the hepatotoxicity of acetaminophen is increased in ethanol-treated animals and the human alcoholic, we have determined the activity of the six available P-450 isozymes in the activation of the drug to give an intermediate which forms a conjugate with reduced glutathione. Isozymes 3a, 4, and 6, all of which are present in significant amounts in the liver microsomes from rabbits chronically admini-stered ethanol, exhibited the highest activities in the reconstituted enzyme system, whereas isozymes 3b and 3c were 10- to 20-fold less effective, and phenobarbital-inducible isozyme 2 was essentially inactive, even in the presence of cytochrome 5. The results obtained thus indicate that induction by ethanol of P-450 isozyme 3a (or a homologous enzyme in other species) may contribute to the toxicity of acetaminophen but that other cytochromes also play a significant role. | en_US |
dc.format.extent | 374091 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Comparison of six rabbit liver cytochrome P-450 isozymes in formation of a reactive metabolite of acetaminophen | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, Medical School The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, Medical School The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, Medical School The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 6838622 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25240/1/0000682.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(83)91789-8 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.