4-Methyleneglutamine synthetase: A new amide synthetase present in germinating peanuts
dc.contributor.author | Winter, Harry C. | en_US |
dc.contributor.author | Su, Ti-Zhi | en_US |
dc.contributor.author | Dekker, Eugene E. | en_US |
dc.date.accessioned | 2006-04-07T18:44:17Z | |
dc.date.available | 2006-04-07T18:44:17Z | |
dc.date.issued | 1983-03-16 | en_US |
dc.identifier.citation | Winter, Harry C., Su, Ti-Zhi, Dekker, Eugene E. (1983/03/16)."4-Methyleneglutamine synthetase: A new amide synthetase present in germinating peanuts." Biochemical and Biophysical Research Communications 111(2): 484-489. <http://hdl.handle.net/2027.42/25265> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4DYVHVW-278/2/a262df04edde22e682f16efbe04271ae | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25265 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6838571&dopt=citation | en_US |
dc.description.abstract | Enzymatic activity which catalyzes the synthesis of 4-methyleneglutamine from 4-methyleneglutamic acid + ammonia was detected in and partially purified from cotyledons of peanut seeds germinated 5 to 7 days. This activity was separated from glutamine and asparagine synthetases by ammonium sulfate precipitation and DEAE-cellulose chromatography. The enzyme is distinct from these other amide synthetases in its substrate specificity, lack of amide/hydroxylamine exchange, and use of ammonium ion as amide donor together with formation of AMP from ATP. The activity is quite labile in solution, but is retained as a precipitate in ammonium sulfate or when frozen in 12.5% glycerol at -77[deg]C. This activity might be responsible for catalyzing the rapid synthesis of 4-methyleneglutamine which occurs in germinating peanuts. | en_US |
dc.format.extent | 404649 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | 4-Methyleneglutamine synthetase: A new amide synthetase present in germinating peanuts | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry The University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry The University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry The University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.identifier.pmid | 6838571 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25265/1/0000708.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(83)90332-7 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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