Evidence for the existence of gonadotropin receptors in the nuclei isolated from rat ovary
dc.contributor.author | Rajendran, K. G. | en_US |
dc.contributor.author | Menon, K. M. J. | en_US |
dc.date.accessioned | 2006-04-07T18:45:25Z | |
dc.date.available | 2006-04-07T18:45:25Z | |
dc.date.issued | 1983-02-28 | en_US |
dc.identifier.citation | Rajendran, K.G., Menon, K.M.J. (1983/02/28)."Evidence for the existence of gonadotropin receptors in the nuclei isolated from rat ovary." Biochemical and Biophysical Research Communications 111(1): 127-134. <http://hdl.handle.net/2027.42/25296> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4G0VTNW-122/2/b123e09dc4a5b1cb90a179a5fa2cafd6 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25296 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6299285&dopt=citation | en_US |
dc.description.abstract | Specific binding of radiolabeled human chorionic gonadotropin (hCG) to nuclei isolated from pseudopregnant rat ovaries was studied. Incubation of cultured luteal cells or isolated nuclei with fluorescein isothiocyanate conjugated hCG showed concentration of fluorescence in the nuclear region. Isolated nuclei exhibited saturable high affinity binding of radiolabeled hCG with an apparent Kd of 3.42 x 10-10 M. The binding was inhibited by increasing concentrations of unlabeled hCG. Under dissociating conditions, the bound hCG was dissociated from the nuclei. However, unlike the plasma membranes, the hCG bound to nuclei was not degraded before dissociation. Radiolabeled hCG bound to the nuclei could also be dissociated by brief exposure to MgCl2 or acidic incubation medium. The bound hCG was not extractable with 4M KCl or 2% Triton X-100. The available evidence suggest that nuclear receptors are distinct from plasma membrane receptors for hCG. | en_US |
dc.format.extent | 862157 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Evidence for the existence of gonadotropin receptors in the nuclei isolated from rat ovary | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Endocrine Laboratory, Departments of Obstetrics and Gynecology and Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Endocrine Laboratory, Departments of Obstetrics and Gynecology and Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 6299285 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25296/1/0000739.pdf | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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