Hexose transport in microvascular endothelial cells cultured from bovine retina
dc.contributor.author | Betz, A. Lorris | en_US |
dc.contributor.author | Bowman, Phillip D. | en_US |
dc.contributor.author | Goldstein, Gary W. | en_US |
dc.date.accessioned | 2006-04-07T18:46:01Z | |
dc.date.available | 2006-04-07T18:46:01Z | |
dc.date.issued | 1983-02 | en_US |
dc.identifier.citation | Betz, A. Lorris, Bowman, Phillip D., Goldstein, Gary W. (1983/02)."Hexose transport in microvascular endothelial cells cultured from bovine retina." Experimental Eye Research 36(2): 269-277. <http://hdl.handle.net/2027.42/25311> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WFD-4BJVVK8-4H/2/4189d3f2f58b4c636cdebe3f9571a18b | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25311 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6337860&dopt=citation | en_US |
dc.description.abstract | The nature of glucose transport at the microvascular blood-retinal barrier was studied using primary cultures of microvascular endothelial cells from bovine retina. Uptake of 3-O-methyl--glucose (3MG), a non-metabolizable glucose analogue, was rapid and equilibrative. 3MG uptake could be inhibited by traditional glucose transport inhibitors such as phloretin, phlorizin and cytochalasin B but not by agents that deplete intracellular ATP (2,4-dinitrophenol) or that abolish the sodium gradient (ouabain). Uptake of 3MG by the cells could be stimulated by preloading with 50 m-glucose, a phenomenon known as counter-transport. Insulin had no effect on 3MG uptake in the cells even after prolonged insulin deprivation. These results demonstrate the existence of a facilitative-diffusion type of glucose transport system in endothelial cells of the retinal microvasculature. Studies with 2-deoxy--glucose demonstrated that transport was not rate limiting for metabolism and that the endothelial cells contain free sugar when exposed to an extracellular sugar concentration in the physiologic range. The presence of this free sugar within the cell is necessary for efficient transendothelial transfer of glucose from blood to retina but it could also provide the basis for capillary damage in diabetes mellitus. | en_US |
dc.format.extent | 1621652 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Hexose transport in microvascular endothelial cells cultured from bovine retina | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Ophthalmology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A.; Department of Neurology, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pediatrics, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A.; Department of Neurology, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pediatrics, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A.; Department of Neurology, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 6337860 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25311/1/0000756.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0014-4835(83)90011-8 | en_US |
dc.identifier.source | Experimental Eye Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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