Improved culture of individual muscle fibres with and without spinal cord explants in a collagen gel
dc.contributor.author | Jay, Jeanne C. | en_US |
dc.contributor.author | Barald, Kate F. | en_US |
dc.date.accessioned | 2006-04-07T18:55:04Z | |
dc.date.available | 2006-04-07T18:55:04Z | |
dc.date.issued | 1985 | en_US |
dc.identifier.citation | Jay, Jeanne C., Barald, Kate F. (1985)."Improved culture of individual muscle fibres with and without spinal cord explants in a collagen gel." Journal of Neuroscience Methods 15(3): 229-234. <http://hdl.handle.net/2027.42/25513> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T04-484M841-2N/2/6c529dfe7566586f3a8b3a0f04fffbf9 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25513 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3005779&dopt=citation | en_US |
dc.description.abstract | Suspension culture of single adult rat flexor digitorum brevis (FDB) muscle fibres in Vitrogen, a purified collagen, on tissue culture plastic or glass with mesh ring supports is superior to culture upon other substrates including collagen-, laminin-, or Vitrogen-coated tissue culture plastic. The Vitrogen gel-fibre mixture which attaches to glass or plastic provides at least 10 times more fibres per dish than does plating fibres on other substrates. Use of Vitrogen gel permits variable plating densities and the production of adequate numbers of cultures for long-term experimental comparisons of acetylcholinesterase (AChE) and rhodamine-[alpha]-bungarotoxin (RBTX) distribution on muscle fibres. Use of 40 [mu]/ml ovotransferrin (OT) instead of chick embryo extract in the culture medium significantly improves long-term survival. Cultured fibres, with or without the addition of ventral spinal cord explants, may also be examined with electrophysiological techniques. | en_US |
dc.format.extent | 446605 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Improved culture of individual muscle fibres with and without spinal cord explants in a collagen gel | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Anatomy and Cell Biology, Program in Neuroscience, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Anatomy and Cell Biology, Program in Neuroscience, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 3005779 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25513/1/0000054.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0165-0270(85)90102-5 | en_US |
dc.identifier.source | Journal of Neuroscience Methods | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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