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Polymyxin B use does not ensure endotoxin-free solution
dc.contributor.authorKluger, Matthew J.en_US
dc.contributor.authorSinger, Rebeccaen_US
dc.contributor.authorEiger, Steven M.en_US
dc.date.accessioned2006-04-07T18:56:09Z
dc.date.available2006-04-07T18:56:09Z
dc.date.issued1985-10-24en_US
dc.identifier.citationKluger, Matthew J., Singer, Rebecca, Eiger, Steven M. (1985/10/24)."Polymyxin B use does not ensure endotoxin-free solution." Journal of Immunological Methods 83(1): 201-207. <http://hdl.handle.net/2027.42/25529>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T2Y-476HN80-28/2/cba7ad3c87559fe1ecc11249317a84e7en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/25529
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2997328&dopt=citationen_US
dc.description.abstractPolymyxin B is often added to in vitro samples to `ensure' that endotoxin activity is removed. We present data, from the standard rabbit pyrogen test and the Limulus amebocyte lysate assay, that polymyxin B bound to a gel support will bind some, but not all, endotoxin. These data, in conjunction with previously published data by Morrison and Curry (1979), indicate that those studies that have relied on polymyxin B to inactivate endotoxin must be re-evaluated.en_US
dc.format.extent339477 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titlePolymyxin B use does not ensure endotoxin-free solutionen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Physiology, The University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationumDepartment of Physiology, The University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationumDepartment of Physiology, The University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A.en_US
dc.identifier.pmid2997328en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/25529/1/0000070.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0022-1759(85)90073-0en_US
dc.identifier.sourceJournal of Immunological Methodsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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