Solubilization of hen brain neurotoxic esterase in dimethylsulfoxide
dc.contributor.author | Schwab, Bradley W. | en_US |
dc.contributor.author | Davis, Cinda-Sue | en_US |
dc.contributor.author | Miller, P. H. | en_US |
dc.contributor.author | Richardson, Rudy J. | en_US |
dc.date.accessioned | 2006-04-07T18:56:23Z | |
dc.date.available | 2006-04-07T18:56:23Z | |
dc.date.issued | 1985-10-15 | en_US |
dc.identifier.citation | Schwab, B. W., Davis, C-S. G., Miller, P. H., Richardson, R. J. (1985/10/15)."Solubilization of hen brain neurotoxic esterase in dimethylsulfoxide." Biochemical and Biophysical Research Communications 132(1): 81-87. <http://hdl.handle.net/2027.42/25532> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4DNHWB6-83/2/ffb88714ff243280b55e747f4c16c4ea | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25532 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4062936&dopt=citation | en_US |
dc.description.abstract | Neurotoxic esterase is the putative site of initiation of organophosphorus-induced neuropathy. The protein is membrane-associated and will thus require solubilization before it can be purified. Its enzymic activity was retained in hen brain microsomes suspended in 10-60% (v/v in water) dimethylsulfoxide and 5-20% dimethylacetamide, but lost in 5-20% 1- and 2-propanol as well as higher concentrations of dimethylsulfoxide. Soluble activity (100,000 x g, 60 min supernatant) was not obtained with dimethylacetamide, but 24% of the recovered activity (67%) was solubilized in 40% dimethylsulfoxide, with retention of its native response to inhibitors. Solvent extraction of active enzyme is of intrinsic interest and expands the options for its purification. | en_US |
dc.format.extent | 427239 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Solubilization of hen brain neurotoxic esterase in dimethylsulfoxide | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Toxicology Program, School of Public Health, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Toxicology Program, School of Public Health, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Toxicology Program, School of Public Health, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Toxicology Program, School of Public Health, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 4062936 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25532/1/0000073.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(85)90991-X | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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