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Solubilization of hen brain neurotoxic esterase in dimethylsulfoxide

dc.contributor.authorSchwab, Bradley W.en_US
dc.contributor.authorDavis, Cinda-Sueen_US
dc.contributor.authorMiller, P. H.en_US
dc.contributor.authorRichardson, Rudy J.en_US
dc.date.accessioned2006-04-07T18:56:23Z
dc.date.available2006-04-07T18:56:23Z
dc.date.issued1985-10-15en_US
dc.identifier.citationSchwab, B. W., Davis, C-S. G., Miller, P. H., Richardson, R. J. (1985/10/15)."Solubilization of hen brain neurotoxic esterase in dimethylsulfoxide." Biochemical and Biophysical Research Communications 132(1): 81-87. <http://hdl.handle.net/2027.42/25532>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WBK-4DNHWB6-83/2/ffb88714ff243280b55e747f4c16c4eaen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/25532
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4062936&dopt=citationen_US
dc.description.abstractNeurotoxic esterase is the putative site of initiation of organophosphorus-induced neuropathy. The protein is membrane-associated and will thus require solubilization before it can be purified. Its enzymic activity was retained in hen brain microsomes suspended in 10-60% (v/v in water) dimethylsulfoxide and 5-20% dimethylacetamide, but lost in 5-20% 1- and 2-propanol as well as higher concentrations of dimethylsulfoxide. Soluble activity (100,000 x g, 60 min supernatant) was not obtained with dimethylacetamide, but 24% of the recovered activity (67%) was solubilized in 40% dimethylsulfoxide, with retention of its native response to inhibitors. Solvent extraction of active enzyme is of intrinsic interest and expands the options for its purification.en_US
dc.format.extent427239 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleSolubilization of hen brain neurotoxic esterase in dimethylsulfoxideen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumToxicology Program, School of Public Health, The University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumToxicology Program, School of Public Health, The University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumToxicology Program, School of Public Health, The University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumToxicology Program, School of Public Health, The University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid4062936en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/25532/1/0000073.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0006-291X(85)90991-Xen_US
dc.identifier.sourceBiochemical and Biophysical Research Communicationsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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