Effect of dietary cholesterol on erythrocyte peroxidant stress in vitro and in vivo
dc.contributor.author | Bereza, Ulana L. | en_US |
dc.contributor.author | Brewer, George J. | en_US |
dc.contributor.author | Hill, Gretchen M. | en_US |
dc.date.accessioned | 2006-04-07T19:02:15Z | |
dc.date.available | 2006-04-07T19:02:15Z | |
dc.date.issued | 1985-07-31 | en_US |
dc.identifier.citation | Bereza, Ulana L., Brewer, George J., Hill, Gretchen M. (1985/07/31)."Effect of dietary cholesterol on erythrocyte peroxidant stress in vitro and in vivo." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 835(3): 434-440. <http://hdl.handle.net/2027.42/25615> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1X-47F6YRC-5V/2/b9e19989cba13ce5cf41a1f107b30140 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25615 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4016140&dopt=citation | en_US |
dc.description.abstract | The effect of dietary variation of plasma cholesterol concentrations on the susceptibility of erythrocytes to in vitro and in vivo peroxidant stress was studied in rats. Malonyldialdehyde, produced in vivo (endogenous malonyldialdehyde) or following in vitro exposure of cells to 10 mM H2O2 (H2O2 malonyldialdehyde, was used as a measure of peroxidant stress. After 5 weeks, the plasma cholesterol concentrations in rats receiving 1.2% cholesterol + 0.6% cholic acid in their diet rose to 6-times that of control rats receiving a diet without added cholesterol; at the same time, erythrocyte H2O2 malonyldialdehyde in the cholesterol-fed rats decreased significantly relative to the control rats. During subsequent exposure of both groups to in vivo peroxidant stress with phenylhydrazine in two separate dose trials, erythrocyte peroxidant stress remained significantly lower in the cholesterol-fed rats: at a dose of 100 [mu]mol/100 g body weight, H2O2 malonyldialdehyde was lower; at a dose of 25 [mu]mol/100 g body weight, both endogenous and H2O2 malonyldialdehyde were lower. Erythrocyte membrane cholesterol concentrations were 12% higher in the cholesterol-fed rats than in controls. The effects of in vivo peroxidant stress on plasma cholesterol were also studied. In vivo peroxidant stress at the higher dose of phenylhydrazine produced a decrease in plasma cholesterol concentrations of control rats. The lower dose had no effect on this group and the plasma cholesterol concentrations were unchanged in the cholesterol-fed rats during both treatments. The data suggest that elevated plasma cholesterol concentrations are protective against erythrocyte peroxidant stress. The mechanism of cholesterol's protective effect is probably mediated through elevated membrane cholesterol concentrations. | en_US |
dc.format.extent | 752448 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Effect of dietary cholesterol on erythrocyte peroxidant stress in vitro and in vivo | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, The University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Human Genetics, The University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Human Genetics, The University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 4016140 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25615/1/0000163.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2760(85)90112-2 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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