Presence of a novel epithelial antigen on rat cerebellar cell lines as detected by a monoclonal antibody
dc.contributor.author | Giotta, G. J. | en_US |
dc.contributor.author | Heitzmann, J. G. | en_US |
dc.contributor.author | Du Bois, Monica | en_US |
dc.contributor.author | Bowman, Phillip D. | en_US |
dc.date.accessioned | 2006-04-07T19:03:03Z | |
dc.date.available | 2006-04-07T19:03:03Z | |
dc.date.issued | 1985-07 | en_US |
dc.identifier.citation | Giotta, G. J., Heitzmann, J. G., DuBois, M., Bowman, P. D. (1985/07)."Presence of a novel epithelial antigen on rat cerebellar cell lines as detected by a monoclonal antibody." Developmental Brain Research 21(1): 61-71. <http://hdl.handle.net/2027.42/25625> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6SYW-4835PKB-3V/2/b0e7c8bd50f589b2a74d8c949a40a595 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25625 | |
dc.description.abstract | We have derived a monoclonal antibody, MCAb 51, following immunization of BALB/c mice with a Rous sarcoma virus-transformed rat cerebellar cell line. When assayed by immunofluorescence on primary rat cerebellar cultures MCAb 51 recognizes only islands of cells with an epitheloid morphology. Double-label immunofluorescence experiments with MCAb 51 and antisera to tetanus toxin, glial fibrillary acidic protein, galactocerebroside and fibronectin reveal that these cells do not appear to be neurons, astrocytes, oligodendrocytes, or fibroblasts, respectively. In contrast, cells from kidney, liver, tongue and choroid plexus epithelium are positive for the antigen. Of 12 Rous sarcoma virus-transformed cell lines, in contrast to 2 out of 9 chemically transformed lines, 11 exhibit the MCAb 51 antigen. These findings demonstrate that MCAb 51 recognizes an epithelial cell surface marker. Possible explanations for the difference in the expression of the antigen on Rous sarcoma virus and chemically transformed neural lines are discussed. | en_US |
dc.format.extent | 874044 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Presence of a novel epithelial antigen on rat cerebellar cell lines as detected by a monoclonal antibody | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics, University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pediatrics, University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationother | Salk Institute, Developmental Biology Laboratory, San Diego, CA 92138, U.S.A. | en_US |
dc.contributor.affiliationother | Salk Institute, Developmental Biology Laboratory, San Diego, CA 92138, U.S.A. | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25625/1/0000175.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0165-3806(85)90023-9 | en_US |
dc.identifier.source | Developmental Brain Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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