Immune induction of human monocyte plasminogen activator. Characteristics of an assay for cell-mediated immunity
dc.contributor.author | Ragsdale, Carol Godoshian | en_US |
dc.contributor.author | Swartz, Kenneth H. | en_US |
dc.contributor.author | Cassidy, James T. | en_US |
dc.date.accessioned | 2006-04-07T19:04:54Z | |
dc.date.available | 2006-04-07T19:04:54Z | |
dc.date.issued | 1985-05-10 | en_US |
dc.identifier.citation | Ragsdale, Carol Godoshian, Swartz, Kenneth H., Cassidy, James T. (1985/05/10)."Immune induction of human monocyte plasminogen activator. Characteristics of an assay for cell-mediated immunity." Journal of Immunological Methods 79(1): 13-26. <http://hdl.handle.net/2027.42/25668> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T2Y-476NCV4-XX/2/e11d2b03c8dec64eaa52ebb98e858e6d | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25668 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2987356&dopt=citation | en_US |
dc.description.abstract | We characterized immunologic induction of monocyte plasminogen activator (PA) to determine whether assay for PA induction reliably detected cell-mediated immunity (CMI). Mononuclear leukocytes (MNL) were incubated in teflon-lined culture tubes for 1-4 days in the presence or absence of phytohemagglutinin-P (PHA), concanavalin A (Con A) or Candida antigen. PA activity of the monocytes in those suspensions was then measured using a micro fibrin plate assay.Monocytes in stimulated MNL had more PA activity than monocytes in unstimulated MNL. Maximal differences between stimulated and unstimulated cells were seen after 2 days of culture. Dose-response studies demonstrated that PA induction occurred at submitogenic concentrations of stimuli. Peak induction was seen using suboptimally mitogenic concentrations of PHA, Con A and Candida antigen. PA induction in response to Candida stimulation corresponded with skin test results. More than 90% of healthy adults tested had positive assays to all stimuli. LPS, in picogram concentrations, induced PA activity in the absence of lymphocytes, but such induction was prevented by polymyxin B.Supernates from activated MNL also induced PA in purified monocytes. This indirect assay of PA induction was less sensitive than direct assay of the MNL. A standard indirect assay for leukocyte inhibitory factor (LIF) was also less sensitive than the direct PA induction assay.The direct PA induction assay is sensitive and convenient and requires small volumes of blood. It may prove valuable in in vitro analysis of cell-mediated immunity in health and disease. | en_US |
dc.format.extent | 698453 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Immune induction of human monocyte plasminogen activator. Characteristics of an assay for cell-mediated immunity | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Rheumatology, Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Division of Rheumatology, Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Rackham Arthritis Unit, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A.; Division of Rheumatology, Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 2987356 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25668/1/0000220.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0022-1759(85)90387-4 | en_US |
dc.identifier.source | Journal of Immunological Methods | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.