Studies on the identity of the heme-binding cysteinyl residue in rabbit liver microsomal cytochrome P-450 isozyme 2

Abstract

The reaction of purified rabbit liver microsomal P-450 isozyme 2 with 4,4'-dithiobis(2-nitrobenzoate) (DTNB) exhibits first order kinetics and results in the modification of a single thiol, but causes no net loss of the native ferrous-carbonyl spectrum. Inclusion of both phospholipid and a tight-binding nitrogenous ligand, 1-benzylimidazole, in the reaction medium produces a burst-phase of DTNB modification, but the stoichiometry remains one thiol modified per polypeptide chain. The site of isozyme 2 rapidly labeled by DTNB and by monobromobimane, a fluorescent reagent for thiol groups, was shown to be Cys152. Results obtained strongly suggest that Cys152 does not provide the proximal thiolate ligand to the heme iron atom. Since Cys152 represents one of the two highly conserved cysteine-containing regions in the P-450 cytochromes, it appears likely that the other region, containing Cys436 in this rabbit cytochrome (corresponding to Cys355 in bacterial P-450 cam, Cys436 in rat P-450 b or e, Cys461 in rat P-450 c, Cys456 in rat P-450 d or mouse isozyme 3, and Cys458 in mouse isozyme 1) is the source of the thiolate ligand to the heme.