Muscarinic cholinergic receptors in human infant forebrain: [3H]Quinuclidinyl benzilate binding in homogenates and quantitative autoradiography in sections
dc.contributor.author | Johnston, Michael V. | en_US |
dc.contributor.author | Silverstein, Faye Sarah | en_US |
dc.contributor.author | Reindel, Fredericko O. | en_US |
dc.contributor.author | Penney, Jr. , John B. | en_US |
dc.contributor.author | Young, Anne B. | en_US |
dc.date.accessioned | 2006-04-07T19:07:07Z | |
dc.date.available | 2006-04-07T19:07:07Z | |
dc.date.issued | 1985-04 | en_US |
dc.identifier.citation | Johnston, Michael V., Silverstein, Fayes S., Reindel, Fredericko O., Penney, Jr., John B., Young, Anne B. (1985/04)."Muscarinic cholinergic receptors in human infant forebrain: [3H]Quinuclidinyl benzilate binding in homogenates and quantitative autoradiography in sections." Developmental Brain Research 19(2): 195-203. <http://hdl.handle.net/2027.42/25719> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6SYW-484M7FH-R/2/84e9ff148587c73137a9f7203460c990 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25719 | |
dc.description.abstract | The ontogeny of muscarinic receptors in human brain was studied by comparing [3H]quinuclidinyl benzilate ([3H]QNB) binding in postmortem tissue from infants 1 week to 3 months of age with binding in adult specimens. Saturation analysis with [3H]QNB and displacement studies with muscarinic antagonists and agonists in tissue homogenates demonstrated that binding sites in the infants' forebrain regions were present in adult or higher than adult concentrations (Bmax). Binding affinity (Kd) and pharmacological characteristics were nearly identical at the two ages. Quantitative receptor autoradiography demonstrated more [3H]QNB binding in the gray matter of infants than adults and revealed a marked difference between the two ages in the laminar distribution of binding sites in neocortex. In contrast to the adult pattern with higher binding in superficial layers 1-3 than in layers 4-6, the distribution in the immature cortex was inverted. These results suggest that muscarinic receptors in infants resemble closely those in mature brain. However, the topography of receptors in the immature neocortex is distinct and they are redistributed in a gradient from inside outward during postnatal development. | en_US |
dc.format.extent | 757873 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Muscarinic cholinergic receptors in human infant forebrain: [3H]Quinuclidinyl benzilate binding in homogenates and quantitative autoradiography in sections | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics, University of Michigan Medical School, Ann Arbor, MI 48104, U.S.A.; Department of Neurology, University of Michigan Medical School, Ann Arbor, MI 48104, U.S.A.; The Center for Human Growth and Development, Ann Arbor, MI 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pediatrics, University of Michigan Medical School, Ann Arbor, MI 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pediatrics, University of Michigan Medical School, Ann Arbor, MI 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan Medical School, Ann Arbor, MI 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan Medical School, Ann Arbor, MI 48104, U.S.A. | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25719/1/0000276.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0165-3806(85)90191-9 | en_US |
dc.identifier.source | Developmental Brain Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.