New spectrophotometric and radiochemical assays for acetyl-CoA: Arylamine N-acetyltransferase applicable to a variety of arylamines
dc.contributor.author | Andres, Herbert H. | en_US |
dc.contributor.author | Klem, Antje J. | en_US |
dc.contributor.author | Szabo, Susan M. | en_US |
dc.contributor.author | Weber, Wendell W. | en_US |
dc.date.accessioned | 2006-04-07T19:08:52Z | |
dc.date.available | 2006-04-07T19:08:52Z | |
dc.date.issued | 1985-03 | en_US |
dc.identifier.citation | Andres, Herbert H., Klem, Antje J., Szabo, Susan M., Weber, Wendell W. (1985/03)."New spectrophotometric and radiochemical assays for acetyl-CoA: Arylamine N-acetyltransferase applicable to a variety of arylamines." Analytical Biochemistry 145(2): 367-375. <http://hdl.handle.net/2027.42/25752> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-4DW2JY5-C8/2/4a00a68d482feea7b563953bc98c5bb5 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25752 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4014668&dopt=citation | en_US |
dc.description.abstract | Simple and sensitive spectrophotometric and radiochemical procedures are described for the assay of acetyl-CoA:arylamine N-acetyltransferase (NAT; EC 2.3.1.5), which catalyzes the reaction acetyl-CoA + arylamine --> N-acetylated arylamine + CoASH. The methods are applicable to crude tissue homogenates and blood lysates. The spectrophotometric assay is characterized by two features: (i) NAT activity is measured by quantifying the disappearance of the arylamine substrate as reflected by decreasing Schiff's base formation with dimethylaminobenzaldehyde. (ii) During the enzymatic reaction, the inhibitory product CoASH is recycled by the system acetyl phosphate/phosphotransactylase to the substrate acetyl-CoA. The radiochemical procedure depends on enzymatic synthesis of [3H]acetyl-CoA in the assay using [3H]acetate, ATP, CoASH, and acetyl-CoA synthetase. NAT activity is measured by quantifying N-[3H]acetylarylamine after separation from [3H]acetate by extraction. Product inhibition by CoASH is prevented in this system by the use of acetyl-CoA synthetase. | en_US |
dc.format.extent | 804222 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | New spectrophotometric and radiochemical assays for acetyl-CoA: Arylamine N-acetyltransferase applicable to a variety of arylamines | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 4014668 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25752/1/0000312.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2697(85)90376-8 | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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