Role of alcohol P-450-oxygenase (APO) in microsomal ethanol oxidation
dc.contributor.author | Koop, Dennis R. | en_US |
dc.contributor.author | Coon, Minor J. | en_US |
dc.date.accessioned | 2006-04-07T19:11:03Z | |
dc.date.available | 2006-04-07T19:11:03Z | |
dc.date.issued | 1985 | en_US |
dc.identifier.citation | Koop, Dennis R., Coon, Minor J. (1985)."Role of alcohol P-450-oxygenase (APO) in microsomal ethanol oxidation." Alcohol 2(1): 23-26. <http://hdl.handle.net/2027.42/25797> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T40-4859SPB-8/2/9df43643af5eb1c829f7aefecc552a06 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25797 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4015835&dopt=citation | en_US |
dc.description.abstract | The form of liver microsomal cytochrome P-450 induced by chronic administration of ethanol to rabbits, designated as P-450 or P-450 isozyme 3a, has been purified to homogeneity as judged by several criteria, including NH2- and COOH-terminal amino acid sequence determination. The reconstituted alcohol-P-450 oxygenase (APO) system containing P-450 and NADPH-cytochrome P-450 reductase catalyzes the oxidation of a variety of primary and secondary alcohols to aldehydes and ketones, including methanol, ethanol, n-propanol, n-butanol, 2-butanol, n-pentanol, and cyclohexanol. Other purified P-450 cytochromes, including isozymes 2, 3b, 3c, 4, and 6, are much less active than P-450 in the oxidation of alcohols. That P-450 functions in ethanol oxidation in liver microsomal membranes as well as in the reconstituted system was shown by immunochemical experiments involving inhibition by sheep anti-P-450 antibodies. We conclude that P-450 is the predominant ethanol-oxidizing cytochrome present after induction by chronic alcohol administration and that the other P-450 cytochromes have low but significant activity in both control and ethanol-induced animals. | en_US |
dc.format.extent | 395181 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Role of alcohol P-450-oxygenase (APO) in microsomal ethanol oxidation | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, Medical School, The University of Michigan, Ann Arbor, MI 48109-0010, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, Medical School, The University of Michigan, Ann Arbor, MI 48109-0010, USA | en_US |
dc.identifier.pmid | 4015835 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25797/1/0000359.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0741-8329(85)90008-4 | en_US |
dc.identifier.source | Alcohol | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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