Combined autoradiographic-immunocytochemical analysis of opioid receptors and opioid peptide neuronal systems in brain
dc.contributor.author | Lewis, Michael E. | en_US |
dc.contributor.author | Khachaturian, Henry | en_US |
dc.contributor.author | Watson, Stanley J. | en_US |
dc.date.accessioned | 2006-04-07T19:11:19Z | |
dc.date.available | 2006-04-07T19:11:19Z | |
dc.date.issued | 1985 | en_US |
dc.identifier.citation | Lewis, Michael E., Khachaturian, Henry, Watson, Stanley J. (1985)."Combined autoradiographic-immunocytochemical analysis of opioid receptors and opioid peptide neuronal systems in brain." Peptides 6(Supplement 1): 37-47. <http://hdl.handle.net/2027.42/25804> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T0M-47STWT2-DM/2/e85e4172db9d25e40978bb29deed8dc3 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25804 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2995943&dopt=citation | en_US |
dc.description.abstract | Using adjacent section autoradiography-immunocytochemistry, the distribution of [3H]naloxone binding sites was studied in relation to neuronal systems containing [Leu]enkephalin, dynorphin A, or [beta]-endorphin immunoreactivity in rat brain. Brain sections from formaldehyde-perfused rats show robust specific binding of [3H]naloxone, the pharmacological ([mu]-like) properties of which appear unaltered. In contrast, specific binding of the [delta] ligand [3H]D-Ala2,D-Leu5-enkephalin was virtually totally eliminated as a result of formaldehyde perfusion. Using adjacent section analysis, we have noted associations between [3H]naloxone binding sites and one, two, or all three opioid systems in different brain regions; however, in some areas, no apparent relationship could be observed. Within regions, the relationship was complex; for example, in caudate-putamen, patches of opioid receptors did not correspond to the distribution of enkephalin immunoreactivity, but there was a correspondence between subcallosal streaks of binding sites and enkephalin. The complexity of the association between [3H]naloxone binding sites and the multiple opioid systems, and previous reports of colocalization of [mu] and [kappa] receptors in rat brain, are inconsistent with a simple-one-to-one relationship between a given opioid precursor and opioid receptor subtype. Instead, since differential processing of the three precursors gives rise to peptides of varying receptor subtype potencies and selectivities, the multiple peptide-receptor relationships may point to a key role of post-translational processing in determining the physiological consequences of opioid neurotransmission. | en_US |
dc.format.extent | 1515341 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Combined autoradiographic-immunocytochemical analysis of opioid receptors and opioid peptide neuronal systems in brain | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.identifier.pmid | 2995943 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25804/1/0000367.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0196-9781(85)90010-5 | en_US |
dc.identifier.source | Peptides | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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