High-performance thin-layer chromatography assay for epoxide hydrolase activity and the determination of phenoxypropane-1,2-diols
dc.contributor.author | Van Den Eeckhout, E. | en_US |
dc.contributor.author | de Moerloose, P. | en_US |
dc.contributor.author | Sinsheimer, Joseph E. | en_US |
dc.date.accessioned | 2006-04-07T19:14:49Z | |
dc.date.available | 2006-04-07T19:14:49Z | |
dc.date.issued | 1985 | en_US |
dc.identifier.citation | van den Eeckhout, E., de Moerloose, P., Sinsheimer, J. E. (1985)."High-performance thin-layer chromatography assay for epoxide hydrolase activity and the determination of phenoxypropane-1,2-diols." Journal of Chromatography A 318(): 343-349. <http://hdl.handle.net/2027.42/25891> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TG8-44BFKY5-XD/2/89721d3d0f2b2df9c92e16bafa788427 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25891 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3980627&dopt=citation | en_US |
dc.description.abstract | A simple, rapid and sensitive high-performance thin-layer chromatographic assay for the determination of epoxide hydrolase activity in rat liver homogenates is described. It is extended to the determination of a series of phenoxypropne-1,2-diols. The hydrolase assay has the advantages of using a readily available substrate, 2,3-epoxypropyl 4-methoxyphenyl ether, of multiple sample application, and of the simultaneous determination of reaction product (diol) as well as unchanged substrate (epoxide). The use of an internal standard, 4-nitroanisole, results in high sensitivity and good reproducibility of the proposed method. The limit of dial detection is 20 pmol. | en_US |
dc.format.extent | 503459 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | High-performance thin-layer chromatography assay for epoxide hydrolase activity and the determination of phenoxypropane-1,2-diols | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan, College of Pharmacy, Ann Arbor, MI 48109 U.S.A. | en_US |
dc.contributor.affiliationother | State University of Ghent, Faculty of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry and Drug Quality Control, Harelbekestraat 72, B-9000 Ghent Belgium | en_US |
dc.contributor.affiliationother | State University of Ghent, Faculty of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry and Drug Quality Control, Harelbekestraat 72, B-9000 Ghent Belgium | en_US |
dc.identifier.pmid | 3980627 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25891/1/0000454.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0021-9673(01)90695-8 | en_US |
dc.identifier.source | Journal of Chromatography A | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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