Pharmacological and anatomical evidence of selective [mu], [delta], and [chi] opioid receptor binding in rat brain
dc.contributor.author | Mansour, Alfred | en_US |
dc.contributor.author | Lewis, Michael E. | en_US |
dc.contributor.author | Khachaturian, Henry | en_US |
dc.contributor.author | Akil, Huda | en_US |
dc.contributor.author | Watson, Stanley J. | en_US |
dc.date.accessioned | 2006-04-07T19:22:45Z | |
dc.date.available | 2006-04-07T19:22:45Z | |
dc.date.issued | 1986-12-03 | en_US |
dc.identifier.citation | Mansour, Alfred, Lewis, Michael E., Khachaturian, Henry, Akil, Huda, Watson, Stanley J. (1986/12/03)."Pharmacological and anatomical evidence of selective [mu], [delta], and [chi] opioid receptor binding in rat brain." Brain Research 399(1): 69-79. <http://hdl.handle.net/2027.42/25947> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6SYR-4840KCR-NS/2/4f20073997490721d293d74c9b93c8cd | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25947 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3026574&dopt=citation | en_US |
dc.description.abstract | While the distribution of opioid receptors can be differentiated in the rat central nervous system, their precise localization has remained controversial, due, in part, to the previous lack of selective ligands and insensitive assaying conditions. The present study analyzed this issue further by examining the receptor selectivity of [3H]DAGO (Tyr--Ala-Gly-MePhe-Gly-ol), [3H]DPDPE (2--penicillamine-5--penicillamine-enkephalin), [3H]DSLET (Tyr--Ser-Gly-Phe-Leu-Thr) and [3H](-)bremazocine, and their suitability in autoradiographically labelling selective subpopulations of opoiod receprtors in rat brain. The results from saturation, competitions, and autoradiographic experiments indicated that the three opioid receptor subtypes can be differentiated in the rat brain and that [3H]-DAGO and [3H]DPDPE selectively labelled [mu] and [delta] binding sites, respectively. In contrast, [3H]DSLET was found to be relatively non-selective, and labelled both [mu] and [delta] sites. [3H]Bremazocine was similarly non-selective in the absence of [mu] and [delta] ligands and labelled all three opioid receptor subtypes. However, in the presence of 100 nM DAGO and DPDPE, concentrations sufficient to saturate the [mu] and [delta] sites, [3H]bremazocine did label [chi] sites selectively. The affinity [3H]bremazocine binding sites showed a unique distribution with relatively dense [chi] labelling in the hypothalamus and median eminence, areas with extremely low [mu] and [delta] binding. These results point to the selectivity, under appropriate conditions, of [3H]DAGO, [3H]DPDPE and [3H]bremazocine and provide evidence for the differential distribution of [mu], [delta], and [chi] opioid receptors in rat brain. | en_US |
dc.format.extent | 1038148 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Pharmacological and anatomical evidence of selective [mu], [delta], and [chi] opioid receptor binding in rat brain | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 3026574 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25947/1/0000010.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-8993(86)90601-3 | en_US |
dc.identifier.source | Brain Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.