Muscarinic receptor-stimulated Ca2+ signaling and inositol lipid metabolism in avian salt gland cells
dc.contributor.author | Snider, R. Michael | en_US |
dc.contributor.author | Roland, Richard M. | en_US |
dc.contributor.author | Lowy, R. Joel | en_US |
dc.contributor.author | Agranoff, Bernard W. | en_US |
dc.contributor.author | Ernst, Stephen A. | en_US |
dc.date.accessioned | 2006-04-07T19:23:54Z | |
dc.date.available | 2006-04-07T19:23:54Z | |
dc.date.issued | 1986-11-28 | en_US |
dc.identifier.citation | Snider, R. Michael, Roland, Richard M., Lowy, R. Joel, Agranoff, Bernard W., Ernst, Stephen A. (1986/11/28)."Muscarinic receptor-stimulated Ca2+ signaling and inositol lipid metabolism in avian salt gland cells." Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 889(2): 216-224. <http://hdl.handle.net/2027.42/25973> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T20-47S6DCV-CJ/2/f6a43c682fa3f3e67e6458e7b13c1904 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25973 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3778948&dopt=citation | en_US |
dc.description.abstract | Activation of muscarinic cholinergic receptors was studied by measuring agonist-stimulated inositol lipid turnover and changes in [Ca2+]i in dissociated salt gland secretory cells. Carbachol stimulation of quin2-loaded cells results in a sustained 4-fold increase in [Ca2+]i, while incorporation of [32P]Pi into phosphatidylinositol (PI) and phosphatidate are similarly increased. [3H]Inositol phosphates, measured in the presence of Li+, increased 13-fold. The stimulated increment in [Ca2+]i required extracellular Ca2+, whereas [3H]inositol phosphate accumulation was independent of external Ca2+. Dose-response curves for carbachol-induced increments in [Ca2+]i, PI labeling, and labeled inositol phosphate release are similar, with EC50 values of 6, 4.5 and 8 [mu]M, respectively. Dissociation constants for atropine vs. the quin2 and phospholipid responses are 0.59 +/- 0.3 nM and 0.48 +/- 0.28 nM, respectively. These cells thus provide a model system for the study of non-exocytotic secretion as a consequence of stimulated inositol lipid turnover. | en_US |
dc.format.extent | 717749 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Muscarinic receptor-stimulated Ca2+ signaling and inositol lipid metabolism in avian salt gland cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48104-1687, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, MI 48104-1687, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, MI 48104-1687, U.S.A. | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48104-1687, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, MI 48104-1687, U.S.A. | en_US |
dc.identifier.pmid | 3778948 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25973/1/0000039.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0167-4889(86)90107-2 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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