Resolution of a common RNA sequencing ambiguity by terminal deoxynucleotidyl transferase
dc.contributor.author | DeBorde, Dan C. | en_US |
dc.contributor.author | Naeve, Clayton W. | en_US |
dc.contributor.author | Louise Herlocher, M. | en_US |
dc.contributor.author | Maassab, Hunein F. | en_US |
dc.date.accessioned | 2006-04-07T19:26:56Z | |
dc.date.available | 2006-04-07T19:26:56Z | |
dc.date.issued | 1986-09 | en_US |
dc.identifier.citation | DeBorde, Dan C., Naeve, Clayton W., Louise Herlocher, M., Maassab, Hunein F. (1986/09)."Resolution of a common RNA sequencing ambiguity by terminal deoxynucleotidyl transferase." Analytical Biochemistry 157(2): 275-282. <http://hdl.handle.net/2027.42/26058> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-4DYVHVH-S/2/e2ac7c0450215a94d4b24b5855319a32 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26058 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2430486&dopt=citation | en_US |
dc.description.abstract | One of the more common ambiguities which arise when using reverse transcriptase and dideoxynucleotide-chain termination to sequence RNA is a radioactive band of cDNA that extends over all four lanes on a sequencing gel. The adjacent sequences both above and below the band are not affected. Assuming then, that these ambiguities are caused by the termination of the DNA polymerase activity of reverse transcriptase for reasons other than the insertion of a dideoxynucleotide in the growing cDNA chain, terminal deoxynucleotidyl transferase should be able to continue to add deoxynucleotides to these products after the sequencing reaction is complete. It does, clearing the improperly terminated cDNA from these pileup sites, revealing the correct sequence. This technique can also be used to identify the template RNA's 5'-terminal base, although far more units of terminal deoxynucleotidyl transferase are required. | en_US |
dc.format.extent | 1572303 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Resolution of a common RNA sequencing ambiguity by terminal deoxynucleotidyl transferase | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Epidemiology, School of Public Health, University of Michigan, 109 Observatory, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Epidemiology, School of Public Health, University of Michigan, 109 Observatory, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Epidemiology, School of Public Health, University of Michigan, 109 Observatory, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101, USA | en_US |
dc.identifier.pmid | 2430486 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26058/1/0000132.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2697(86)90626-3 | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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