Isolation of a cDNA coding for human galactosyltransferase
dc.contributor.author | Appert, Hubert E. | en_US |
dc.contributor.author | Rutherford, Thomas J. | en_US |
dc.contributor.author | Tarr, George E. | en_US |
dc.contributor.author | Wiest, Jon S. | en_US |
dc.contributor.author | Thomford, Neil R. | en_US |
dc.contributor.author | McCorquodale, D. James | en_US |
dc.date.accessioned | 2006-04-07T19:27:17Z | |
dc.date.available | 2006-04-07T19:27:17Z | |
dc.date.issued | 1986-08-29 | en_US |
dc.identifier.citation | Appert, Hubert E., Rutherford, Thomas J., Tarr, George E., Wiest, Jon S., Thomford, Neil R., McCorquodale, D. James (1986/08/29)."Isolation of a cDNA coding for human galactosyltransferase." Biochemical and Biophysical Research Communications 139(1): 163-168. <http://hdl.handle.net/2027.42/26068> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4G0VNMJ-N5/2/bc88cb5406b21e8edd9c53956c4a5597 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26068 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3094506&dopt=citation | en_US |
dc.description.abstract | Human milk galactosyltransferase (EC 2.4.1.22) was purified to homogeneity using affinity chromatography. Edman degradation was used to determine the amino acid sequences of eight peptide fragments isolated from the purified enzyme. A 60-mer "optimal" oligonucleotide probe that corresponded to the amino acid sequence of one of the galactosyltransferase peptide fragments was constructed and used to screen a [lambda]gt10 cDNA library. Two hybridization-positive recombinant phages, each with a 1.7 Kbp insert, were detected among 3 x 106 recombinant [lambda]gt10 phages. Sequencing of one of the cDNA inserts revealed a 783 bp galactosyltransferase coding sequence. The remainder of the sequence corresponded to the 3'-region of the mRNA downstream from the termination codon. | en_US |
dc.format.extent | 359714 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Isolation of a cDNA coding for human galactosyltransferase | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The University of Michigan, Department of Biochemistry Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Medical College of Ohio at Toledo, Department of Surgery, C.S. 10008, Toledo, Ohio 43699, USA | en_US |
dc.contributor.affiliationother | Medical College of Ohio at Toledo, Department of Surgery, C.S. 10008, Toledo, Ohio 43699, USA; Medical College of Ohio at Toledo, Department of Biochemistry C.S. 10008, Toledo, Ohio 43699, USA. | en_US |
dc.contributor.affiliationother | Medical College of Ohio at Toledo, Department of Biochemistry C.S. 10008, Toledo, Ohio 43699, USA | en_US |
dc.contributor.affiliationother | Medical College of Ohio at Toledo, Department of Surgery, C.S. 10008, Toledo, Ohio 43699, USA | en_US |
dc.contributor.affiliationother | Medical College of Ohio at Toledo, Department of Biochemistry C.S. 10008, Toledo, Ohio 43699, USA | en_US |
dc.identifier.pmid | 3094506 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26068/1/0000142.pdf | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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