Carbohydrate binding properties of the stinging nettle (Urtica dioica) rhizome lectin
dc.contributor.author | Shibuya, Naoto | en_US |
dc.contributor.author | Goldstein, Irwin J. | en_US |
dc.contributor.author | Shafer, Jules A. | en_US |
dc.contributor.author | Peumans, Willy J. | en_US |
dc.contributor.author | Broekaert, Willem F. | en_US |
dc.date.accessioned | 2006-04-07T19:27:23Z | |
dc.date.available | 2006-04-07T19:27:23Z | |
dc.date.issued | 1986-08-15 | en_US |
dc.identifier.citation | Shibuya, Naoto, Goldstein, Irwin J., Shafer, Jules A., Peumans, Willey J., Broekaert, Willem F. (1986/08/15)."Carbohydrate binding properties of the stinging nettle (Urtica dioica) rhizome lectin." Archives of Biochemistry and Biophysics 249(1): 215-224. <http://hdl.handle.net/2027.42/26071> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DPBXJX-D5/2/4e7d1242a21ef2a40e84beafabd0e4ea | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26071 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3740853&dopt=citation | en_US |
dc.description.abstract | The interaction of the stinging nettle rhizome lectin (UDA) with carbohydrates was studied by using the techniques of quantitative precipitation, hapten inhibition, equilibrium dialysis, and uv difference spectroscopy. The Carbohydrate binding site of UDA was determined to be complementary to an N,N',N"-triacetylchitotriose unit and proposed to consist of three subsites, each of which has a slightly different binding specificity. UDA also has a hydrophobic interacting region adjacent to the carbohydrate binding site. Equilibrium dialysis and uv difference spectroscopy revealed that UDA has two carbohydrate binding sites per molecule consisting of a single polypeptide chain. These binding sites either have intrinsically different affinities for ligand molecules, or they may display negative cooperativity toward ligand binding. | en_US |
dc.format.extent | 849195 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Carbohydrate binding properties of the stinging nettle (Urtica dioica) rhizome lectin | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Laboratorium voor Plantenbiochemie, KU Leuven, Kardinaal Mercierlaan, 92, B-3030, Leuven, Belgium | en_US |
dc.contributor.affiliationother | Laboratorium voor Plantenbiochemie, KU Leuven, Kardinaal Mercierlaan, 92, B-3030, Leuven, Belgium | en_US |
dc.identifier.pmid | 3740853 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26071/1/0000145.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(86)90577-1 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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