Isolation of galactosyltransferase from human milk and the determination of its N-terminal amino acid sequence
dc.contributor.author | Appert, Hubert E. | en_US |
dc.contributor.author | Rutherford, Thomas J. | en_US |
dc.contributor.author | Tarr, George E. | en_US |
dc.contributor.author | Thomford, Neil R. | en_US |
dc.contributor.author | McCorquodale, D. James | en_US |
dc.date.accessioned | 2006-04-07T19:28:29Z | |
dc.date.available | 2006-04-07T19:28:29Z | |
dc.date.issued | 1986-07-16 | en_US |
dc.identifier.citation | Appert, Hubert E., Rutherford, Thomas J., Tarr, George E., Thomford, Neil R., McCorquodale, D. James (1986/07/16)."Isolation of galactosyltransferase from human milk and the determination of its N-terminal amino acid sequence." Biochemical and Biophysical Research Communications 138(1): 224-229. <http://hdl.handle.net/2027.42/26099> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4F031HH-MF/2/0425ebd71e3f1c71c5d1427bf9b77539 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26099 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3091013&dopt=citation | en_US |
dc.description.abstract | Galactosyltransferase (EC 2.4.1.22), purified to homogeneity from human milk by affinity chromatography, had an apparent molecular weight of 53,000 as determined by denaturing polyacrylamide gel electrophoresis. Subtration of the estimated contribution of the oligosaccharide portion of the molecule leaves a Mr of 47,000. An N-terminal amino acid sequence analysis of the isolated protein revealed a sequence similar to that found near the 5' end of a cDNA clone isolated by Shaper et al (11), which encodes a 35,500 molecular weight protein. Either the molecular weight of galactosyltransferase, has been overestimated, or a discrepancy exists between the actual molecular weight of galactosyltransferase and that predicted by the bovine cDNA clone isolated by Shaper et al (11). | en_US |
dc.format.extent | 1175715 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Isolation of galactosyltransferase from human milk and the determination of its N-terminal amino acid sequence | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan, Department of Biochemistry, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Medical College of Ohio at Toledo, Department of Surgery C.S. 10008, Toledo, Ohio 43699, USA | en_US |
dc.contributor.affiliationother | Medical College of Ohio at Toledo, Department of Biochemistry C.S. 10008, Toledo, Ohio 43699, USA | en_US |
dc.contributor.affiliationother | Medical College of Ohio at Toledo, Department of Surgery C.S. 10008, Toledo, Ohio 43699, USA | en_US |
dc.contributor.affiliationother | Medical College of Ohio at Toledo, Department of Biochemistry C.S. 10008, Toledo, Ohio 43699, USA | en_US |
dc.identifier.pmid | 3091013 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26099/1/0000175.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(86)90269-X | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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