EPR of a novel high-spin component in activated hydrogenase from Desulfovibrio vulgaris (Hildenborough)
dc.contributor.author | Hagen, Wilfred R. | en_US |
dc.contributor.author | van Berkel-Arts, A. | en_US |
dc.contributor.author | Kruse-Wolters, K. M. | en_US |
dc.contributor.author | Dunham, William Richard | en_US |
dc.contributor.author | Veeger, Cornelis | en_US |
dc.date.accessioned | 2006-04-07T19:30:58Z | |
dc.date.available | 2006-04-07T19:30:58Z | |
dc.date.issued | 1986-05-26 | en_US |
dc.identifier.citation | Hagen, W. R., van Berkel-Arts, A., Kruse-Wolters, K. M., Dunham, W. R., Veeger, C. (1986/05/26)."EPR of a novel high-spin component in activated hydrogenase from Desulfovibrio vulgaris (Hildenborough)." FEBS Letters 201(1): 158-162. <http://hdl.handle.net/2027.42/26169> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T36-44KP5CM-15/2/8532f8b09be8df33c33a164258444378 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26169 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3011503&dopt=citation | en_US |
dc.description.abstract | The EPR of reoxidized hydrogenase from Desulfovibrio vulgaris (H.) has been reinvestigated. In contrast to other workers [(1984) Proc. Natl. Acad. Sci. USA 81, 3728-3732] we find the axial signal with g = 2.06; 2.01 to be only a minor component of concentration 0.03 spin/mol. In the spectrum of fully active reoxidized enzyme this signal is overshadowed by a rhombic signal (0.1 spin/mol) with g = 2.11; 2.05; 2.00 reminiscent of the only signal found for other oxidized bidirectional hydrogenases. In addition, a novel signal has been detected with geff = 5.0 which, under the assumptions that S = 2 and |[Delta]ms|= 2, quantitates to roughly one spin/mol. Ethylene glycol affects the relative intensity of the different signals. It is suggested that O2 sensitization parallels a spin-state transition of an iron-sulfur cluster. | en_US |
dc.format.extent | 579204 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | EPR of a novel high-spin component in activated hydrogenase from Desulfovibrio vulgaris (Hildenborough) | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Biophysics Research Division, Institute of Science and Technology, The University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationother | Department of Biochemistry, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands | en_US |
dc.contributor.affiliationother | Department of Biochemistry, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands | en_US |
dc.contributor.affiliationother | Department of Biochemistry, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands | en_US |
dc.contributor.affiliationother | Department of Biochemistry, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands | en_US |
dc.identifier.pmid | 3011503 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26169/1/0000246.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0014-5793(86)80590-7 | en_US |
dc.identifier.source | FEBS Letters | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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