Subcellular distribution and properties of acyl/alkyl dihydroxyacetone phosphate reductase in rodent livers
dc.contributor.author | Ghosh, Mridul K. | en_US |
dc.contributor.author | Hajra, Amiya K. | en_US |
dc.date.accessioned | 2006-04-07T19:34:08Z | |
dc.date.available | 2006-04-07T19:34:08Z | |
dc.date.issued | 1986-03 | en_US |
dc.identifier.citation | Ghosh, Mridul K., Hajra, Amiya K. (1986/03)."Subcellular distribution and properties of acyl/alkyl dihydroxyacetone phosphate reductase in rodent livers." Archives of Biochemistry and Biophysics 245(2): 523-530. <http://hdl.handle.net/2027.42/26255> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DPC11R-109/2/77f46b3300d7d39e312aaaf77937ad69 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26255 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3954368&dopt=citation | en_US |
dc.description.abstract | On subcellular fractionation, the enzyme acyl/alkyl dihydroxyacetone phosphate (DHAP) reductase (EC 1.1.1.101) in guinea pig and rat liver was found to be present in both the light mitochondrial (L) and microsomal fractions. By using metrizamide density gradient centrifugation, it was shown that the alkyl DHAP reductase activity in the "L" fraction is localized mainly in peroxisomes. From the distribution of the marker enzymes it was calculated that about two-thirds of the liver reductase activity is in the peroxisomes and the rest in the microsomes. The properties of this enzyme in peroxisomes and microsomes are similar with respect to heat inactivation, pH optima, sensitivity to trypsin, and inhibition by NADP+ and acyl CoA. The enzyme activity in the peroxisomes and microsomes from mouse liver is increased to the same extent by chronically feeding the animals clofibrate, a hypolipidemic drug. The kinetic properties of this enzyme in these two different organelles are also similar. From these results it is concluded that the same enzyme is present in two different subcellular compartments of liver. | en_US |
dc.format.extent | 699420 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Subcellular distribution and properties of acyl/alkyl dihydroxyacetone phosphate reductase in rodent livers | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory, Mental Health Research Institute, and Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory, Mental Health Research Institute, and Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.identifier.pmid | 3954368 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26255/1/0000336.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(86)90245-6 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.