Isolation and characterization of a novel cytochrome P-450-like pseudogene
dc.contributor.author | Zaphiropoulos, Peter G. | en_US |
dc.contributor.author | Folk, William R. | en_US |
dc.contributor.author | Coon, Minor J. | en_US |
dc.date.accessioned | 2006-04-07T19:35:18Z | |
dc.date.available | 2006-04-07T19:35:18Z | |
dc.date.issued | 1986-01-29 | en_US |
dc.identifier.citation | Zaphiropoulos, Peter G., Folk, William R., Coon, Minor J. (1986/01/29)."Isolation and characterization of a novel cytochrome P-450-like pseudogene." Biochemical and Biophysical Research Communications 134(2): 499-505. <http://hdl.handle.net/2027.42/26288> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4G3D5KD-NM/2/4e043683d3ef189898d486a45ff72f81 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26288 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3004450&dopt=citation | en_US |
dc.description.abstract | SummaryA rabbit liver P-450-like pseudogene has been isolated from a [lambda] phage genomic library. Sequence analysis revealed structural homology with respect to the rat P-450b and P-450e genes as well as a similar intron-exon organization. A 5'-proximal TATA box-like sequence and two 3'-distal putative polyadenylation signals were identified, and all putative intronexon boundaries except at the 3'-splice site of intron 2 were found to follow the GT/AG rule. With allowance for apparent deletions and insertions, the structural homology of the amino acid sequence deduced from the pseudogene with respect to rabbit P-450 isozyme 2 is lower for exons 1 through 4 (18-28%) than for exons 5 through 9 (42-65%). S1 nuclease mapping showed that mRNAs complementary to the DNA sequence of exon 9 are expressed. However, due to the alterations in the pseudogene, it appears that functional P-450 would not be produced from such mRNAs. | en_US |
dc.format.extent | 737411 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Isolation and characterization of a novel cytochrome P-450-like pseudogene | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry Medical School, The University of Michigan Ann Arbor, Michigan 48109 USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry Medical School, The University of Michigan Ann Arbor, Michigan 48109 USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry Medical School, The University of Michigan Ann Arbor, Michigan 48109 USA | en_US |
dc.identifier.pmid | 3004450 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26288/1/0000373.pdf | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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