Simultaneous determination of thymine and 5-bromouracil in DNA hydrolysates using gas chromatography--mass spectrometry with selected-ion monitoring
dc.contributor.author | Stetson, Philip L. | en_US |
dc.contributor.author | Maybaum, Jonathan | en_US |
dc.contributor.author | Shukla, Umesh A. | en_US |
dc.contributor.author | Ensminger, William D. | en_US |
dc.date.accessioned | 2006-04-07T19:35:56Z | |
dc.date.available | 2006-04-07T19:35:56Z | |
dc.date.issued | 1986 | en_US |
dc.identifier.citation | Stetson, Philip L., Maybaum, Jonathan, Shukla, Umesh A., Ensminger, William D. (1986)."Simultaneous determination of thymine and 5-bromouracil in DNA hydrolysates using gas chromatography--mass spectrometry with selected-ion monitoring." Journal of Chromatography B: Biomedical Sciences and Applications 375(): 1-9. <http://hdl.handle.net/2027.42/26306> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TG9-44V418R-R/2/72639ee39376ab112b9b3ab72316135e | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26306 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3958104&dopt=citation | en_US |
dc.description.abstract | The gas chromatographic--mass spectrometric method using selected-ion monitoring (GC--MS--SIM) described here quantitatively determines the amount of DNA thymine replacement by 5-bromouracil (BU) after exposure to 5-bromo-2'-deoxyuridine (BUDR) in as few as 105 cells. DNA is extracted, enzymatically hydrolyzed, the nucleic acid bases (with added internal standards, 5-iodouracil and 5-chlorouracil) are extracted into ethyl acetate, concentrated and derivatized with bis(trimethylsilyl)trifluoroacetamide. Thymine and BU are then quantitated by GC--MS--SIM. Response is linear to thymine over the range of 100-2000 ng per sample and BU of 1.3-52 ng per sample with a coefficient of variation of less than 10% and an accuracy for seeded samples within 8% of theoretical value. With V79 cells in culture, exposure to increasing BUDR concentrations (0.03- 1.0 [mu]M) results in increasing thymine substitution by BU over a range of 1-28%. Other important applications of this technique are mentioned. | en_US |
dc.format.extent | 556042 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Simultaneous determination of thymine and 5-bromouracil in DNA hydrolysates using gas chromatography--mass spectrometry with selected-ion monitoring | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Upjohn Center for Clinical Pharmacology, University of Michigan, Ann Arbor, MI 48109 U.S.A. | en_US |
dc.contributor.affiliationum | Upjohn Center for Clinical Pharmacology, University of Michigan, Ann Arbor, MI 48109 U.S.A. | en_US |
dc.contributor.affiliationum | College of Pharmacy, University of Michigan, Ann Arbor, MI 48109 U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Pharmacology and Internal Medicine, University of Michigan, Ann Arbor, MI 48109 U.S.A. | en_US |
dc.identifier.pmid | 3958104 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26306/1/0000391.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0378-4347(00)83686-5 | en_US |
dc.identifier.source | Journal of Chromatography B: Biomedical Sciences and Applications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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