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Direct identification of small sequence changes in chromosomal DNA
dc.contributor.authorHuibregtse, Jon M.en_US
dc.contributor.authorEngelke, David R.en_US
dc.date.accessioned2006-04-07T19:37:52Z
dc.date.available2006-04-07T19:37:52Z
dc.date.issued1986en_US
dc.identifier.citationHuibregtse, Jon M., Engelke, David R. (1986)."Direct identification of small sequence changes in chromosomal DNA." Gene 44(1): 151-158. <http://hdl.handle.net/2027.42/26360>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T39-47PH4R4-G8/2/b68685958901069957aa1ca5bf23ec53en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/26360
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2429900&dopt=citationen_US
dc.description.abstractDideoxynucleotide chain termination sequencing has been applied directly to genomic DNA templates by annealing radiolabeled oligodeoxynucleotide primers to unique sites in total yeast DNA and extending with avian myoblastosis virus (AMV) reverse transcriptase. The technique is used here to confirm the introduction of selectively altered tRNA genes into the Saccharomyces cerevisiae genome by gene replacement.en_US
dc.format.extent764959 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleDirect identification of small sequence changes in chromosomal DNAen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelGeneticsen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, The University of Michigan, Ann Arbor, MI 48109, U.S.A. Tel. (313)763-0641en_US
dc.contributor.affiliationumDepartment of Biological Chemistry, The University of Michigan, Ann Arbor, MI 48109, U.S.A. Tel. (313)763-0641en_US
dc.identifier.pmid2429900en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/26360/1/0000447.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0378-1119(86)90056-9en_US
dc.identifier.sourceGeneen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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