Immobilization and assay of low-molecular-weight phosphomannosyl receptor in multiwell plates
dc.contributor.author | Distler, Jack J. | en_US |
dc.contributor.author | Patel, Rita | en_US |
dc.contributor.author | Jourdian, George W. | en_US |
dc.date.accessioned | 2006-04-07T19:47:31Z | |
dc.date.available | 2006-04-07T19:47:31Z | |
dc.date.issued | 1987-10 | en_US |
dc.identifier.citation | Distler, Jack J., Patel, Rita, Jourdian, George W. (1987/10)."Immobilization and assay of low-molecular-weight phosphomannosyl receptor in multiwell plates." Analytical Biochemistry 166(1): 65-71. <http://hdl.handle.net/2027.42/26547> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-4DYTNJ8-1J4/2/6ce97a465ee54a2e7193f5223a816a96 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26547 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2823641&dopt=citation | en_US |
dc.description.abstract | A novel sensitive binding assay for quantitation of a low-molecular-weight phosphomannosyl receptor (41-46 K) was devised. The receptor was immobilized by immunochemical means in the wells of polystyrene multiwell plates. The lysosomal enzyme ligand, testicular [beta]-galactosidase, was added and receptor-bound [beta]-galactosidase was measured by conventional colorimetric analysis using p-nitrophenyl [beta]-galactoside as substrate. Inhibitors of the binding of [beta]-galactosidase to the receptor were removed prior to addition of [beta]-galactosidase and did not interfere with the assay. Low-molecular-weight phosphomannosyl receptor was readily quantitated in the range of 4 to 100 ng of receptor protein. Binding of [beta]-galactosidase to the receptor was specifically inhibited by 5 m mannose 6-phosphate. The receptor exhibited optimum binding of [beta]-galactosidase at pH 5.7 and was saturated with [beta]-galactosidase at 320 munits/ml in the presence of 20 m MnCl2. The requirement for MnCl2 was greatly diminished at higher concentrations of [beta]-galactosidase. Application of the assay procedure to the quantitation of the low-molecular-weight phosphomannosyl receptor in mammalian tissues is discussed. | en_US |
dc.format.extent | 533822 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Immobilization and assay of low-molecular-weight phosphomannosyl receptor in multiwell plates | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA. | en_US |
dc.identifier.pmid | 2823641 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26547/1/0000086.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2697(87)90546-X | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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