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Characterization of a mutant polyoma that expresses in F9 embryonal carcinoma cells: Morphology, tumorigenicity, and restriction enzyme analysis

dc.contributor.authorLehman, John M.en_US
dc.contributor.authorMcMahon, Jillen_US
dc.contributor.authorHales, Karenen_US
dc.contributor.authorTrevor, Katrinaen_US
dc.date.accessioned2006-04-07T19:49:56Z
dc.date.available2006-04-07T19:49:56Z
dc.date.issued1987-08en_US
dc.identifier.citationLehman, John M., McMahon, Jill, Hales, Karen, Trevor, Katrina (1987/08)."Characterization of a mutant polyoma that expresses in F9 embryonal carcinoma cells: Morphology, tumorigenicity, and restriction enzyme analysis." Experimental and Molecular Pathology 47(1): 59-68. <http://hdl.handle.net/2027.42/26615>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WFB-4C4NY45-B3/2/95b50de5afa05998fc641d087ebdb7d3en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/26615
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3038594&dopt=citationen_US
dc.description.abstractA mutant polyoma virus (TT340), which replicates in F9 embryonal carcinoma (EC) cells and contains 2500 base pairs (bp) of additional DNA located in the early noncoding region of the genome, was analyzed to determine the DNA origin of the mutant insertion. Two fragments, representing repeated units of the 2500-bp insert, were isolated from TT340, labeled, and hybridized to the parental wild-type viral DNA. A BglI 500-bp unit, of which there are approximately five copies within the 2500-bp insert, contains sequences homologous to regions on the early and late side of the viral origin of replication. A HpaII 400-bp repeated fragment shows homology to sequences on the early side with little hybridization to the late side. Removal of the 2500-bp insert results in the loss of infectivity on F9 EC cells but not on 3T6 or mouse embryo fibroblasts. Insertion of the BglI 500-bp repeat element into wild-type DNA at the BglI site allows replication of the constructed virus in F9 cells. The mutant virions were tumorigenic in newborn Syrian hamsters and the morphology of the virus was that of wild-type as assayed by electron microscopy.en_US
dc.format.extent758485 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleCharacterization of a mutant polyoma that expresses in F9 embryonal carcinoma cells: Morphology, tumorigenicity, and restriction enzyme analysisen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPathologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan School of Medicine, Ann Arbor, Michigan, USAen_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan School of Medicine, Ann Arbor, Michigan, USAen_US
dc.contributor.affiliationotherDepartment of Pathology, University of Colorado School of Medicine, Denver, Colorado, USA; Department of Microbiology and Immunology, Albany Medical College, Albany, New York 12208, USAen_US
dc.contributor.affiliationotherLa Jolla Cancer Research Foundation, La Jolla, California, USAen_US
dc.identifier.pmid3038594en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/26615/1/0000156.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0014-4800(87)90007-4en_US
dc.identifier.sourceExperimental and Molecular Pathologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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