Genetics of cold-adapted B/Ann Arbor/1/66 influenza virus reassortants: the acidic polymerase (PA) protein gene confers temperature sensitivity and attenuated virulence
dc.contributor.author | Donabedian, Armen M. | en_US |
dc.contributor.author | DeBorde, Dan C. | en_US |
dc.contributor.author | Maassab, Hunein F. | en_US |
dc.date.accessioned | 2006-04-07T19:49:58Z | |
dc.date.available | 2006-04-07T19:49:58Z | |
dc.date.issued | 1987-08 | en_US |
dc.identifier.citation | Donabedian, Armen M., DeBorde, Dan C., Maassab, Hunein F. (1987/08)."Genetics of cold-adapted B/Ann Arbor/1/66 influenza virus reassortants: the acidic polymerase (PA) protein gene confers temperature sensitivity and attenuated virulence." Microbial Pathogenesis 3(2): 97-108. <http://hdl.handle.net/2027.42/26616> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WN6-4C82FM5-4V/2/9a9bd3551f92bd9f2662605c40a4f10c | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26616 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3504545&dopt=citation | en_US |
dc.description.abstract | The cold-adapted B/Ann Arbor/1/66 influenza virus (ca B/AA/1/66) expresses temperature-sensitive (ts), cold-adapted (ca) and attenuation phenotypes. Reassortants which inherit one or more genes from ca B/AA/1/66 and all other genes from a virulent, wild-type influenza virus, B/Houston/1732/76, were produced and evaluated in order to identify the gene(s) responsible for the ts, ca and attenuation phenotypes. Only reassortants which inherited the PA gene from ca B/AA/1/66 expressed the ts phenotype in MDCK cells at 39 [deg]C. None of the reassortants tested expressed the ca phenotype in embryonated eggs at 25 [deg]C. The virulence of several reassortants was evaluated in ferrets. Inheritance of the PA gene from ca B/AA/1/66 was correlated with significant febrile attentuation and the apparent restriction of viral replication in the lower respiratory tract. Isolation of a virulent, non-ts revertant virus inheriting only the PA gene from ca B/AA/1/66 established a direct relationship between expression of the ts phenotype and attenuated virulence. Evidence for the contribution of at least one other gene from ca B/AA/1/66 to attenuation was observed. Thus, based on the methods used to determine reassortant gene compositions, these results indicate that the PA gene is primarily responsible for attenuation of ca B/AA/1/66 and its reassortants. | en_US |
dc.format.extent | 1851877 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Genetics of cold-adapted B/Ann Arbor/1/66 influenza virus reassortants: the acidic polymerase (PA) protein gene confers temperature sensitivity and attenuated virulence | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Epidemiology, The University of Michigan, 109 Observatory, St, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Epidemiology, The University of Michigan, 109 Observatory, St, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Epidemiology, The University of Michigan, 109 Observatory, St, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.identifier.pmid | 3504545 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26616/1/0000157.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0882-4010(87)90068-4 | en_US |
dc.identifier.source | Microbial Pathogenesis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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