Monoclonal antibody (5G6.4) against ovarian carcinoma shows inhibition of in vitro colony formation
dc.contributor.author | Rodriguez-Rodriguez, Lorna | en_US |
dc.contributor.author | Liebert, Monica | en_US |
dc.contributor.author | Natale, Ronald B. | en_US |
dc.contributor.author | Wahl, Richard L. | en_US |
dc.date.accessioned | 2006-04-07T19:51:51Z | |
dc.date.available | 2006-04-07T19:51:51Z | |
dc.date.issued | 1987-07 | en_US |
dc.identifier.citation | Rodriguez-Rodriguez, Lorna, Liebert, Monica, Natale, Ronald, Wahl, Richard (1987/07)."Monoclonal antibody (5G6.4) against ovarian carcinoma shows inhibition of in vitro colony formation." Gynecologic Oncology 27(3): 382-388. <http://hdl.handle.net/2027.42/26666> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WG6-4C5H0F6-166/2/5d61b28a4ebc31145b09118b574e78f8 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26666 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3623234&dopt=citation | en_US |
dc.description.abstract | Monoclonal antibodies (MAbs) have the potential for diagnosis and therapy of cancer. 5G6.4 is a MAb of the IgG2a class which was produced by immunization of BALB/c mice with human ovarian carcinoma (Ov Ca) cells. To further characterize 5G6.4, its effect on cell growth was tested using a human Ov Ca cell line established in our laboratory. A clonogenic assay was set up in 48-well plates in a double agar system. The cells were plated and 5G6.4 was added at different concentrations. Control plates consisted of cells with media without MAb. Negative control plates were also prepared using the same concentrations of an isotype-matched antimelanoma MAb, 225.28s. Colony formation (CF) was reduced to 50% or less of control with increasing amounts of 5G6.4 up to 50 [mu]g/ml. Although CF was still depressed at concentrations above 50 [mu]g/ml, the inhibition did not follow a directly proportional line; instead, it followed a bell-shaped curve. Plates with the control MAb, 225.28s, did not show this response. Similar results were obtained with cells from malignant Ov Ca ascites in the same clonogenic assay. Our study suggests that in the evaluation of the in vitro effect of MAb on growth, the concentration of MAb is crucial and may not show a linear response and that 5G6.4 may have a direct therapeutic effect by blocking the growth of Ov Ca cells. 5G6.4 is presently under study for therapy in an animal model. | en_US |
dc.format.extent | 457709 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Monoclonal antibody (5G6.4) against ovarian carcinoma shows inhibition of in vitro colony formation | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Otolaryngology | en_US |
dc.subject.hlbsecondlevel | Ophthalmology | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbsecondlevel | Obstetrics and Gynecology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Obstetrics and Gynecology, Division of Gynecological Oncology, University of Michigan, Ann Arbor, Michigan 48109-0028, U.S.A. | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, University of Michigan, Ann Arbor, Michigan 48109-0028, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Medical Oncology, University of Michigan, Ann Arbor, Michigan 48109-0028, U.S.A. | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, University of Michigan, Ann Arbor, Michigan 48109-0028, U.S.A. | en_US |
dc.identifier.pmid | 3623234 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26666/1/0000210.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0090-8258(87)90263-0 | en_US |
dc.identifier.source | Gynecologic Oncology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.