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Enzymatic fluorometric assay for myo-inositol trisphosphate
dc.contributor.authorShayman, James A.en_US
dc.contributor.authorMorrison, Aubrey R.en_US
dc.contributor.authorLowry, Oliver H.en_US
dc.date.accessioned2006-04-07T19:54:00Z
dc.date.available2006-04-07T19:54:00Z
dc.date.issued1987-05-01en_US
dc.identifier.citationShayman, James A., Morrison, Aubrey R., Lowry, Oliver H. (1987/05/01)."Enzymatic fluorometric assay for myo-inositol trisphosphate." Analytical Biochemistry 162(2): 562-568. <http://hdl.handle.net/2027.42/26721>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DV0BP2-8F/2/9a1c53e24adc1288ed9bd65ae7c2a5daen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/26721
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3496810&dopt=citationen_US
dc.description.abstractThe determination of myo-inositol trisphosphate by an enzymatic fluorometric assay is presented. The method involves the acid extraction of water-soluble inositol polyphosphates followed by separation by anion-exchange chromatography. Samples are subsequently neutralized by passage over a Dowex Cl- resin and elution with lithium chloride. Samples are then desalted with ethanol. Following dephosphorylation with alkaline phosphatase, free myo-inositol is measured enzymatically via the NAD-dependent oxidation to scyllo-inosose with myo-inositol dehydrogenase. The efficiency of recovery, assay specificity, and an application to the measurement of inositol polyphosphates in hormone-stimulated tissue are discussed.en_US
dc.format.extent591903 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleEnzymatic fluorometric assay for myo-inositol trisphosphateen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Medicine, The University of Michigan Medical Center, Ann Arbor, Michigan 48109-0364, USA; Department of Medicine, Washington University Medical School, St. Louis, Missouri 63110, USA; Department of Pharmacology, Washington University Medical School, St. Louis, Missouri 63110, USA.en_US
dc.contributor.affiliationumDepartment of Medicine, Washington University Medical School, St. Louis, Missouri 63110, USA; Department of Pharmacology, Washington University Medical School, St. Louis, Missouri 63110, USA; Department of Medicine, The University of Michigan Medical Center, Ann Arbor, Michigan 48109-0364, USA.en_US
dc.contributor.affiliationumDepartment of Medicine, Washington University Medical School, St. Louis, Missouri 63110, USA; Department of Pharmacology, Washington University Medical School, St. Louis, Missouri 63110, USA; Department of Medicine, The University of Michigan Medical Center, Ann Arbor, Michigan 48109-0364, USA.en_US
dc.identifier.pmid3496810en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/26721/1/0000271.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(87)90434-9en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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