Evaluation of methods for the estimation of mutation rates in cultured mammalian cell populations
dc.contributor.author | Li, I-Chian | en_US |
dc.contributor.author | Chu, Ernest H. Y. | en_US |
dc.date.accessioned | 2006-04-07T19:55:10Z | |
dc.date.available | 2006-04-07T19:55:10Z | |
dc.date.issued | 1987-04 | en_US |
dc.identifier.citation | Li, I-chian, Chu, E. H. Y. (1987/04)."Evaluation of methods for the estimation of mutation rates in cultured mammalian cell populations." Mutation Research Letters 190(4): 281-287. <http://hdl.handle.net/2027.42/26754> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B73H4-482RCNP-2T/2/a2e7fbd307027a741f72197cea96ee2b | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26754 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3550454&dopt=citation | en_US |
dc.description.abstract | A systematic comparison of 5 different statistical methods for the estimation of mutation rate ([mu]) in cultured Chinese hamster V79 cells is presented. Fluctuation tests were performed with several large batches of parallel cell cultures each allowed to grow for a different length of time in order to reach different population size (Nt). Based on Lea and Coulson's theoretical distribution, a comparison has been made between the experimental data and the expected distribution of the number of ouabain-resistant mutants per culture in these hamster cell populations. The sum of squared deviation between the observed and expected values, or SSD, was used as a means of the adequacy of the estimation method; the method which gives the smallest SSD is regarded as the best one for the estimation of [mu]. Our results show that when Nt is small, the occurrence of mutation is infrequent, and SSDs from different methods are similar. However, when Nt is large, there is a great discrepancy of the SSD values, suggesting a preference of using the maximum likelihood method, the P0 method, the median method, the upper quartile method and the mean method, in that order, for the estimation of [mu]. The order of preference is correlated with estimation efficiencies. Depending on the size of Nt and the method used, the estimated [mu] may vary up to more than 3-fold. At a large Nt, the [mu] obtained from the maximum likelihood method is very precise. This suggests the importance of choosing an appropriate Nt as well as method for the estimation of [mu]. | en_US |
dc.format.extent | 493493 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Evaluation of methods for the estimation of mutation rates in cultured mammalian cell populations | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Medicine, Medical College, National Cheng Kung University, Tainan 70101, Taiwan; Department of Human Genetics, Medical School, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Human Genetics, Medical School, University of Michigan, Ann Arbor, MI 48109, U.S.A.; Department of Medicine, Medical College, National Cheng Kung University, Tainan 70101, Taiwan | en_US |
dc.identifier.pmid | 3550454 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26754/1/0000306.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0165-7992(87)90010-8 | en_US |
dc.identifier.source | Mutation Research Letters | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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