Changes in protein kinase C activity are associated with the differentiation of Friend erythroleukemia cells
dc.contributor.author | Balazovich, Kenneth J. | en_US |
dc.contributor.author | Portnow, Douglas | en_US |
dc.contributor.author | Boxer, Laurence A. | en_US |
dc.contributor.author | Prochownik, Edward V. | en_US |
dc.date.accessioned | 2006-04-07T19:56:49Z | |
dc.date.available | 2006-04-07T19:56:49Z | |
dc.date.issued | 1987-02-18 | en_US |
dc.identifier.citation | Balazovich, Kenneth J., Portnow, Douglas, Boxer, Laurence A., Prochownik, Edward V. (1987/02/18)."Changes in protein kinase C activity are associated with the differentiation of Friend erythroleukemia cells." Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 927(2): 247-255. <http://hdl.handle.net/2027.42/26799> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T20-47S646B-H9/2/522a1217d918f61a24f5b7c1c7066e15 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26799 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3469000&dopt=citation | en_US |
dc.description.abstract | We investigated the activity and cellular distribution of protein kinase C during the dimethylsulfoxide (DMSO) and hypoxanthine-induced differentiation of Friend murine erythroleukemia cells. Most of the cellular protein kinase C activity was found in the soluble fraction of unstimulated Friend cells. Within 15 min of the addition of DMSO or hypoxanthine, protein kinase C underwent a dramatic and prolonged reversal of this distribution which was accompanied by a gradual decline in total cellular protein kinase C activity over the ensuing 5 days. The loss of total activity was found to be dose dependent although maximal translocation from soluble to insoluble components occurred at even lower concentrations of the inducers tested. Two clones of Friend cells, selected for their failure to differentiate in response to DMSO, showed alterations in protein kinase C activity and/or distribution following DMSO addition when compared to wild-type Friend cells. These data show that different inducers of Friend cell differentiation have similar effects on cellular protein kinase C, that the protein kinase C changes accompanying this process are immediate but prolonged, and that changes in protein kinase C activity and distribution are associated with Friend cell differentiation. | en_US |
dc.format.extent | 713170 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Changes in protein kinase C activity are associated with the differentiation of Friend erythroleukemia cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics, Section of Pediatric Hematology-Oncology and the Committee on Cellular and Molecular Biology, University of Michigan Medical School, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pediatrics, Section of Pediatric Hematology-Oncology and the Committee on Cellular and Molecular Biology, University of Michigan Medical School, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pediatrics, Section of Pediatric Hematology-Oncology and the Committee on Cellular and Molecular Biology, University of Michigan Medical School, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pediatrics, Section of Pediatric Hematology-Oncology and the Committee on Cellular and Molecular Biology, University of Michigan Medical School, Ann Arbor, MI, U.S.A. | en_US |
dc.identifier.pmid | 3469000 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26799/1/0000355.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0167-4889(87)90141-8 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.