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Analysis of bromodeoxyuridine incorporation into DNA: Comparison of gas chromatographic/mass spectrometric, CsCl gradient sedimentation, and specific radioactivity methods
dc.contributor.authorMaybaum, Jonathanen_US
dc.contributor.authorKott, Matthew G.en_US
dc.contributor.authorJohnson, Norma J.en_US
dc.contributor.authorEnsminger, William D.en_US
dc.contributor.authorStetson, Philip L.en_US
dc.date.accessioned2006-04-07T19:56:53Z
dc.date.available2006-04-07T19:56:53Z
dc.date.issued1987-02-15en_US
dc.identifier.citationMaybaum, Jonathan, Kott, Matthew G., Johnson, Norma J., Ensminger, William D., Stetson, Philip L. (1987/02/15)."Analysis of bromodeoxyuridine incorporation into DNA: Comparison of gas chromatographic/mass spectrometric, CsCl gradient sedimentation, and specific radioactivity methods." Analytical Biochemistry 161(1): 164-171. <http://hdl.handle.net/2027.42/26801>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DYTNJ8-B8/2/8b04aaccdb2671c2494f35eb5be0452den_US
dc.identifier.urihttps://hdl.handle.net/2027.42/26801
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3578781&dopt=citationen_US
dc.description.abstractA sensitive new method for the quantitation of 5-bromodeoxyuridine (BrdUrd) incorporated into DNA by GC/MS analysis of enzymatically released Thy and bromouracil (BrUra) is presented. The hydrolysis procedure was characterized and found to give uniform results when sample size was 1-10 [mu]g DNA and incubation time for DNA digestion was between 40 min and 16 h. Samples of DNA containing 3H-labeled BrdUrd were analyzed in parallel by the GC/MS technique and by specific radioactivity and buoyant density measurements, in order to compare the three methods. The GC/MS procedure gave values for percentage replacement of Thy by BrUra which were higher than those obtained by specific activity and lower than those obtained by buoyant density. This GC/MS method can detect 1% replacement in a 1-[mu]g DNA sample, equivalent to approximately 105 cells or 0.1 mg tissue, and will permit sensitive and quantitative analysis of the presence of this chemotherapeutic agent/radiosensitizer in cellular DNA from biopsy samples of normal or tumor tissue.en_US
dc.format.extent704910 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleAnalysis of bromodeoxyuridine incorporation into DNA: Comparison of gas chromatographic/mass spectrometric, CsCl gradient sedimentation, and specific radioactivity methodsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0504, USA.en_US
dc.contributor.affiliationumDepartment of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0504, USAen_US
dc.contributor.affiliationumDepartment of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109-0504, USAen_US
dc.contributor.affiliationumDepartment of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109-0504, USAen_US
dc.contributor.affiliationumDepartment of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0504, USAen_US
dc.identifier.pmid3578781en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/26801/1/0000357.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(87)90667-1en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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