Identification of gastrin molecular variants in gastrinoma syndrome
dc.contributor.author | Kothary, Piyush C. | en_US |
dc.contributor.author | Mahoney, Walter C. | en_US |
dc.contributor.author | Vinik, Aaron I. | en_US |
dc.date.accessioned | 2006-04-07T19:57:29Z | |
dc.date.available | 2006-04-07T19:57:29Z | |
dc.date.issued | 1987-02 | en_US |
dc.identifier.citation | Kothary, Piyush C., Mahoney, Walter C., Vinik, Aaron I. (1987/02)."Identification of gastrin molecular variants in gastrinoma syndrome." Regulatory Peptides 17(2): 71-84. <http://hdl.handle.net/2027.42/26818> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T0S-47TG51K-BN/2/06f3af68f9374d581593cbc28079346d | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26818 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3554401&dopt=citation | en_US |
dc.description.abstract | The molecular species of gastrin in the circulation and in tumor extracts were studied in two groups of patients: (1) with benign gastrinoma and (2) with gastrinoma with liver metastases. Radioimmunoassays (RIAs) and immunoaffinity chromatography for the amino (NH2)- and amidated COOH-terminus of gastrin-17 (antiserum G17) and the NH2-terminus of gastrin-34 (antiserum G34) were employed. In both benign and metastatic tumors the molecular forms of gastrin in boiling water extracts measured by the gastrin-17 NH2- and COOH-terminal assays were similar. In addition to a molecular component resembling the amidated gastrin-17, there were also significant amounts of larger molecular weight (mol. wt.) forms. The larger mol. wt. forms absorbed by the NH2-terminus of G17 antiserum corresponded to the COOH-terminus-extended forms of gastrin-17. Furthermore, larger mol. wt. gastrins immunopurified by antiserum to the NH2-terminus of gastrin-34 corresponded to gastrin-34 extended molecules. Sera of patients with liver metastases had higher concentrations of the NH2-terminal of gastrin-17 whereas sera of patients with benign gastrinoma contained predominantly gastrins detected by the COOH-terminal assay. These results suggest that: (a) there are differences in the molecular pattern of gastrin in the circulation of patients with benign and metastatic gastrinomas; (b) gastrins which are fully processed with carboxy-terminal amidation predominate in the circulation of patients with benign gastrinoma; and (c) gastrins containing the gastrin-17 and COOH-terminally extended gastrin-17 and gastrin-34 precursor molecules occur in high concentration in the circulation of gastrinoma patients with metastases to the liver. | en_US |
dc.format.extent | 765673 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Identification of gastrin molecular variants in gastrinoma syndrome | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Surgery and Internal Medicine (The Division of Endocrinology and Metabolism), University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Surgery and Internal Medicine (The Division of Endocrinology and Metabolism), University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationother | University of Minnesota, Department of Genetics and Cell Biology, St. Paul, MN 55108, U.S.A.; ImmunoNuclear Corporation, Stillwater, MN 55082, U.S.A. | en_US |
dc.identifier.pmid | 3554401 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26818/1/0000376.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0167-0115(87)90014-0 | en_US |
dc.identifier.source | Regulatory Peptides | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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